Mostrar el registro sencillo del ítem
The sf32 unique gene of spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a non-essential gene that could be involved in nucleocapsid organization in occlusion-derived virions
dc.creator | Beperet Arive, Inés | es_ES |
dc.creator | Barrera Cubillos, Gloria Patricia | es_ES |
dc.creator | Simón de Goñi, Oihane | es_ES |
dc.creator | Williams, Trevor | es_ES |
dc.creator | López Ferber, Miguel | es_ES |
dc.creator | Gasmi, Laila | es_ES |
dc.creator | Herrero, Salvador | es_ES |
dc.creator | Caballero Murillo, Primitivo | es_ES |
dc.date.accessioned | 2018-09-06T12:20:19Z | |
dc.date.available | 2018-09-06T12:20:19Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/2454/30547 | |
dc.description.abstract | A recombinant virus lacking the sf32 gene (Sf32null), unique to the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), was generated by homologous recombination from a bacmid comprising the complete viral genome (Sfbac). Transcriptional analysis revealed that sf32 is an early gene. Occlusion bodies (OBs) of Sf32null contained 62% more genomic DNA than viruses containing the sf32 gene, Sfbac and Sf32null-repair, although Sf32null DNA was three-fold less infective when injected in vivo. Sf32null OBs were 18% larger in diameter and contained 17% more nucleocapsids within ODVs than those of Sfbac. No significant differences were detected in OB pathogenicity (50% lethal concentration), speed-of-kill or budded virus production in vivo. In contrast, the production of OBs/larva was reduced by 39% in insects infected by Sf32null compared to those infected by Sfbac. The SF32 predicted protein sequence showed homology (25% identity, 44% similarity) to two adhesion proteins from Streptococcus pyogenes and a single N-mirystoylation site was predicted. We conclude that SF32 is a non-essential protein that could be involved in nucleocapsid organization during ODV assembly and occlusion, resulting in increased numbers of nucleocapsids within ODVs. | en |
dc.description.sponsorship | This study received financial support from the Spanish Ministry for Science and Technology (AGL2008-05456-C03-01, AGL2011-30352-C02-01). I.B. received a CSIC studentship. | en |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Public Library of Science | en |
dc.relation.ispartof | Plos One, 8(10): e77683 | en |
dc.rights | © 2013 Beperet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | |
dc.subject | Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) | en |
dc.subject | sf32 | en |
dc.subject | Nucleocapsid organization | en |
dc.subject | Occlusion-derived virions | en |
dc.title | The sf32 unique gene of spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a non-essential gene that could be involved in nucleocapsid organization in occlusion-derived virions | en |
dc.type | info:eu-repo/semantics/article | en |
dc.type | Artículo / Artikulua | es |
dc.contributor.department | Producción Agraria | es_ES |
dc.contributor.department | Nekazaritza Ekoizpena | eu |
dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.identifier.doi | 10.1371/journal.pone.0077683 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//AGL2011-30352-C02-01/ES/ | en |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0077683 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |