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Non-additive transcriptional profiles underlie dikaryotic superiority in Pleurotus ostreatus laccase activity
dc.creator | Castanera Andrés, Raúl | es_ES |
dc.creator | Omarini, Alejandra | es_ES |
dc.creator | Santoyo Santos, Francisco | es_ES |
dc.creator | Pérez Garrido, María Gumersinda | es_ES |
dc.creator | Pisabarro de Lucas, Gerardo | es_ES |
dc.creator | Ramírez Nasto, Lucía | es_ES |
dc.date.accessioned | 2018-09-06T12:20:21Z | |
dc.date.available | 2018-09-06T12:20:21Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/2454/30551 | |
dc.description.abstract | Background: The basidiomycete Pleurotus ostreatus is an efficient producer of laccases, a group of enzymes appreciated for their use in multiple industrial processes. The aim of this study was to reveal the molecular basis of the superiority of laccase production by dikaryotic strains compared to their parental monokaryons. Methodology/Principal Findings: We bred and studied a set of dikaryotic strains starting from a meiotic population of monokaryons. We then completely characterised the laccase allelic composition, the laccase gene expression and activity profiles in the dikaryotic strain N001, in two of its meiotic full-sib monokaryons and in the dikaryon formed from their mating. Conclusions/Significance: Our results suggested that the dikaryotic superiority observed in laccase activity was due to nonadditive transcriptional increases in lacc6 and lacc10 genes. Furthermore, the expression of these genes was divergent in glucose- vs. lignocellulose-supplemented media and was highly correlated to the detected extracellular laccase activity. Moreover, the expression profile of lacc2 in the dikaryotic strains was affected by its allelic composition, indicating a putative single locus heterozygous advantage. | en |
dc.description.sponsorship | This work was supported by funds obtained from AGL2011-30495 of the Spanish National Research Plan; Bioethanol-Euroinnova, the Autonomous Government of Navarre; and by additional institutional support from the Public University of Navarre. | en |
dc.format.mimetype | application/pdf | en |
dc.format.mimetype | application/zip | en |
dc.language.iso | eng | en |
dc.publisher | Public Library of Science | en |
dc.relation.ispartof | Plos One, 8(9): e73282 | en |
dc.rights | © 2013 Castanera et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | |
dc.subject | Pleurotus ostreatus | en |
dc.subject | Laccases | en |
dc.subject | Dikaryotic strains | en |
dc.title | Non-additive transcriptional profiles underlie dikaryotic superiority in Pleurotus ostreatus laccase activity | en |
dc.type | info:eu-repo/semantics/article | en |
dc.type | Artículo / Artikulua | es |
dc.contributor.department | Producción Agraria | es_ES |
dc.contributor.department | Nekazaritza Ekoizpena | eu |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.identifier.doi | 10.1371/journal.pone.0073282 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//AGL2011-30495/ES/ | en |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0073282 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
dc.contributor.funder | Gobierno de Navarra / Nafarroako Gobernua | es |
dc.contributor.funder | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |