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dc.creatorBaroja Fernández, Edurnees_ES
dc.creatorMuñoz Pérez, Francisco Josées_ES
dc.creatorLi, Junes_ES
dc.creatorBahaji, Abdellatifes_ES
dc.creatorAlmagro Zabalza, Goizederes_ES
dc.creatorMontero Macarro, Manueles_ES
dc.creatorEtxeberria, Edes_ES
dc.creatorHidalgo Cruz, Maitees_ES
dc.creatorSesma Pascual, María Teresaes_ES
dc.creatorPozueta Romero, Javieres_ES
dc.date.accessioned2018-12-28T09:14:04Z
dc.date.available2018-12-28T09:14:04Z
dc.date.issued2011
dc.identifier.issn0027-8424 (Print)
dc.identifier.issn1091-6490 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31885
dc.description.abstractSucrose synthase (SUS) catalyzes the reversible conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate-glucose and fructose. In Arabidopsis, a multigene family encodes six SUS (SUS1-6) isoforms. The involvement of SUS in the synthesis of UDP-glucose and ADP-glucose linked to Arabidopsis cellulose and starch biosynthesis, respectively, has been questioned by Barratt et al. [(2009) Proc Natl Acad Sci USA 106:13124–13129], who showed that (i) SUS activity in wild type (WT) leaves is too low to account for normal rate of starch accumulation in Arabidopsis, and (ii) different organs of the sus1/sus2/sus3/sus4 SUS mutant impaired in SUS activity accumulate WT levels of ADP-glucose, UDP-glucose, cellulose and starch. However, these authors assayed SUS activity under unfavorable pH conditions for the reaction. By using favorable pH conditions for assaying SUS activity, in this work we show that SUS activity in the cleavage direction is sufficient to support normal rate of starch accumulation in WT leaves. We also demonstrate that sus1/sus2/sus3/sus4 leaves display WT SUS5 and SUS6 expression levels, whereas leaves of the sus5/sus6 mutant display WT SUS1–4 expression levels. Furthermore, we show that SUS activity in leaves and stems of the sus1/sus2/sus3/sus4 and sus5/sus6 plants is ~85% of that of WT leaves, which can support normal cellulose and starch biosynthesis. The overall data disprove Barratt et al. (2009) claims, and are consistent with the possible involvement of SUS in cellulose and starch biosynthesis in Arabidopsis.en
dc.description.sponsorshipThis research was partially supported by the Grant BIO2010-18239 from the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain), and by Iden Biotechnology S.L. G.A. acknowledges a fellowship from the Public University of Navarra.en
dc.format.extent6 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherNational Academy of Sciencesen
dc.relation.ispartofPNAS, January 3, 2012 109 (1) 321-326en
dc.subjectCarbohydrate metabolismen
dc.subjectSink strengthen
dc.titleSucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch productionen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB - Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1073/pnas.1117099109
dc.relation.publisherversionhttps://doi.org/10.1073/pnas.1117099109
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes


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