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dc.creatorMoreno Bruna, Beatrizes_ES
dc.creatorBaroja Fernández, Edurnees_ES
dc.creatorMuñoz Pérez, Francisco Josées_ES
dc.creatorBastarrica Berasategui, Ainaraes_ES
dc.creatorZandueta Criado, Aitores_ES
dc.creatorRodríguez López, Milagroses_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.creatorAkazawa, Takashies_ES
dc.creatorPozueta Romero, Javieres_ES
dc.identifier.issn0027-8424 (Print)
dc.identifier.issn1091-6490 (Electronic)
dc.description.abstractAn adenosine diphosphate sugar pyrophosphatase (ASPPase, EC has been characterized by using Escherichia coli. This enzyme, whose activities in the cell are inversely correlated with the intracellular glycogen content and the glucose concentration in the culture medium, hydrolyzes ADP-glucose, the precursor molecule of glycogen biosynthesis. ASPPase was purified to apparent homogeneity (over 3,000-fold), and sequence analyses revealed that it is a member of the ubiquitously distributed group of nucleotide pyrophosphatases designated as ‘‘nudix’’ hydrolases. Insertional mutagenesis experiments leading to the inactivation of the ASPPase encoding gene, aspP, produced cells with marginally low enzymatic activities and higher glycogen content than wildtype bacteria. aspP was cloned into an expression vector and introduced into E. coli. Transformed cells were shown to contain a dramatically reduced amount of glycogen, as compared with the untransformed bacteria. No pleiotropic changes in the bacterial growth occurred in both the aspP-overexpressing and aspP-deficient strains. The overall results pinpoint the reaction catalyzed by ASPPase as a potential step of regulating glycogen biosynthesis in E. coli.en
dc.description.sponsorshipT.A. sincerely appreciates the support from the Spanish Ministry of Culture and Education.en
dc.format.extent5 p.
dc.publisherNational Academy of Sciencesen
dc.relation.ispartofPNAS July 3, 2001 98 (14) 8128-8132en
dc.rights© 2001 The National Academy of Sciencesen
dc.subjectAdenosine diphosphate sugar pyrophosphataseen
dc.subjectEcherichia colien
dc.subjectGlycogen biosynthesisen
dc.titleAdenosine diphosphate sugar pyrophosphatase prevents glycogen biosynthesis in Escherichia colien
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.type.versionVersión publicada / Argitaratu den bertsioaes

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