Stability of a Spodoptera frugiperda nucleopolyhedrovirus deletion recombinant during serial passage in insects
Fecha
2009Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.01894-09
Resumen
The stabilities of the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) complete genome
bacmid (Sfbac) and a deletion recombinant (Sf29null) in which the Sf29 gene was replaced by a kanamycin
resistance cassette were determined during sequential rounds of per os infection in insect larvae. The Sf29 gene
is a viral factor that determines the number of virions in occlusion bodies (OB ...
[++]
The stabilities of the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) complete genome
bacmid (Sfbac) and a deletion recombinant (Sf29null) in which the Sf29 gene was replaced by a kanamycin
resistance cassette were determined during sequential rounds of per os infection in insect larvae. The Sf29 gene
is a viral factor that determines the number of virions in occlusion bodies (OBs). The Sf29null bacmid virus
was able to recover the Sf29 gene during passage. After the third passage (P3) of Sf29null bacmid OBs, the
population was observed to reach an equilibrium involving a mixture of those with a kanamycin resistance
cassette and those with the Sf29 gene. The biological activity of Sf29null bacmid OBs at P3 was similar to that
of Sfbac OBs. The recovered gene in the Sf29null virus was 98 to 100% homologous to the Sf29 genes of different
SfMNPV genotypes. Reverse transcription-PCR analysis of uninoculated S. frugiperda larvae confirmed the
expression of the SfMNPV ie-0 and Sf29 genes, indicating that the insect colony harbors a covert SfMNPV
infection. Additionally, the nonessential bacterial artificial chromosome vector was spontaneously deleted from
both viral genomes upon passage in insects. [--]
Materias
Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV),
Serial passage
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, Feb. 2010, Vol. 76, No. 3, p. 803–809
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This study received financial support from the Spanish Ministry
for Science and Technology projects AGL2005-07909-CO3-01 and
AGL2008-05456-CO3-01/AGR. O.S. received a Spanish Ministry of
Education and Culture postdoctoral fellowship.