Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
Fecha
2012Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.03021-12
Resumen
A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic
composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a
high proportion of large-deletion genotypes that lack genes that are essential for viral replication or transmission. To determine
the likely origins of ...
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A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic
composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a
high proportion of large-deletion genotypes that lack genes that are essential for viral replication or transmission. To determine
the likely origins of such genotypically similar population structures, we performed genomic and functional analyses of these
genotypes. The homology of nucleotides in the deleted regions was as high as 79%, similar to those of other colinear genomic
regions, although some SfMNPV genes were not present in SeMNPV. In addition, no potential consensus sequences were shared
between the deletion flanking sequences. These results indicate an evolutionary mechanism that independently generates and
sustains deletion mutants within each virus population. Functional analyses using different proportions of complete and deletion
genotypes were performed with the two viruses in mixtures of occlusion bodies (OBs) or co-occluded virions. Ratios greater
than 3:1 of complete/deletion genotypes resulted in reduced pathogenicity (expressed as median lethal dose), but there were no
significant changes in the speed of kill. In contrast, OB yields increased only in the 1:1 mixture. The three phenotypic traits analyzed
provide a broader picture of the functional significance of the most extensively deleted SeMNPV genotype and contribute
toward the elucidation of the role of such mutants in baculovirus populations. [--]
Materias
Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV),
Spodoptera frugiperda MNPV (SfMNPV),
Occlusion bodies (OBs)
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, February 2013 Volume 79 Number 4, p. 1118–1125
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This study received financial support from the Spanish Ministry for
Science and Technology (AGL2008-05456-C03-01).
A.S. received a predoctoral fellowship from the Spanish Ministry of
Education and Culture.