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dc.creatorToledo Arana, Alejandroes_ES
dc.creatorValle Turrillas, Jaionees_ES
dc.creatorSolano Goñi, Cristinaes_ES
dc.creatorArrizubieta Balerdi, María Jesúses_ES
dc.creatorCucarella, Carmees_ES
dc.creatorLamata, Martaes_ES
dc.creatorAmorena Zabalza, Beatrizes_ES
dc.creatorLeiva, Josées_ES
dc.creatorPenadés, José R.es_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.date.accessioned2019-01-10T08:27:59Z
dc.date.available2019-01-10T08:27:59Z
dc.date.issued2001
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31949
dc.description.abstractThe enterococcal surface protein, Esp, is a high-molecular-weight surface protein of unknown function whose frequency is significantly increased among infection-derived Enterococcus faecalis isolates. In this work, a global structural similarity was found between Bap, a biofilm-associated protein of Staphylococcus aureus, and Esp. Analysis of the relationship between the presence of the Esp-encoding gene (esp) and the biofilm formation capacity in E. faecalis demonstrated that the presence of the esp gene is highly associated (P < 0.0001) with the capacity of E. faecalis to form a biofilm on a polystyrene surface, since 93.5% of the E. faecalis esp-positive isolates were capable of forming a biofilm. Moreover, none of the E. faecalis esp-deficient isolates were biofilm producers. Depending on the E. faecalis isolate, insertional mutagenesis of esp caused either a complete loss of the biofilm formation phenotype or no apparent phenotypic defect. Complementation studies revealed that Esp expression in an E. faecalis esp-deficient strain promoted primary attachment and biofilm formation on polystyrene and polyvinyl chloride plastic from urine collection bags. Together, these results demonstrate that (i) biofilm formation capacity is widespread among clinical E. faecalis isolates, (ii) the biofilm formation capacity is restricted to the E. faecalis strains harboring esp, and (iii) Esp promotes primary attachment and biofilm formation of E. faecalis on abiotic surfaces.en
dc.description.sponsorshipThis work was supported by grant BIO99-0285 from the Comisión Interministerial de Ciencia y Tecnología and grants from the Cardenal Herrera-CEU University and from the Departamento de Educación y Cultura del Gobierno de Navarra.en
dc.format.extent8 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, Oct. 2001, Vol. 67, No. 10, p. 4538–4545en
dc.rights© 2001, American Society for Microbiology. All Rights Reserved.en
dc.subjectEnterococcal surface proteinen
dc.subjectEnterococcus faecalisen
dc.titleThe enterococcal surface protein, Esp, is involved in Enterococcus faecalis biofilm formationen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.contributor.departmentIdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/aem.67.10.4538-4545.2001
dc.relation.publisherversionhttps://doi.org/10.1128/aem.67.10.4538-4545.2001
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes


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