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Diffusional properties of methanogenic granular sludge: 1H NMR characterization
dc.creator | Lens, Piet N. L. | es_ES |
dc.creator | Gastesi Barasoain, Rakel | es_ES |
dc.creator | Vergeldt, Frank | es_ES |
dc.creator | Van Aelst, Adriaan C. | es_ES |
dc.creator | Pisabarro de Lucas, Gerardo | es_ES |
dc.creator | Van As, Henk | es_ES |
dc.date.accessioned | 2019-01-10T08:28:00Z | |
dc.date.available | 2019-01-10T08:28:00Z | |
dc.date.issued | 2003 | |
dc.identifier.issn | 0099-2240 (Print) | |
dc.identifier.issn | 1098-5336 (Electronic) | |
dc.identifier.uri | https://hdl.handle.net/2454/31952 | |
dc.description.abstract | The diffusive properties of anaerobic methanogenic and sulfidogenic aggregates present in wastewater treatment bioreactors were studied using diffusion analysis by relaxation time-separated pulsed-field gradient nuclear magnetic resonance (NMR) spectroscopy and NMR imaging. NMR spectroscopy measurements were performed at 22°C with 10 ml of granular sludge at a magnetic field strength of 0.5 T (20 MHz resonance frequency for protons). Self-diffusion coefficients of H2O in the investigated series of mesophilic aggregates were found to be 51 to 78% lower than the self-diffusion coefficient of free water. Interestingly, self-diffusion coefficients of H2O were independent of the aggregate size for the size fractions investigated. Diffusional transport occurred faster in aggregates growing under nutrient-rich conditions (e.g., the bottom of a reactor) or at high (55°C) temperatures than in aggregates cultivated in nutrient-poor conditions or at low (10°C) temperatures. Exposure of aggregates to 2.5% glutaraldehyde or heat (70 or 90°C for 30 min) modified the diffusional transport up to 20%. In contrast, deactivation of aggregates by HgCl2 did not affect the H2O self-diffusion coefficient in aggregates. Analysis of NMR images of a single aggregate shows that methanogenic aggregates possess a spin-spin relaxation time and self-diffusion coefficient distribution, which are due to both physical (porosity) and chemical (metal sulfide precipitates) factors. | en |
dc.description.sponsorship | This research was supported by a TMR Marie Curie fellowship (ERBFMBICT950250) and the Human Capital and Mobility EU Large Scale Facility, Wageningen NMR Centre (ERBCHGECT940061). | en |
dc.format.extent | 6 p. | |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | American Society for Microbiology | en |
dc.relation.ispartof | Applied and Environmental Microbiology, Nov. 2003, Vol. 69, No. 11, p. 6644–6649 | en |
dc.rights | © 2003, American Society for Microbiology. All Rights Reserved. | en |
dc.subject | Nuclear magnetic resonance (NMR) | en |
dc.subject | Diffusional properties | en |
dc.subject | Methanogenic granular sludge | en |
dc.title | Diffusional properties of methanogenic granular sludge: 1H NMR characterization | en |
dc.type | info:eu-repo/semantics/article | en |
dc.type | Artículo / Artikulua | es |
dc.contributor.department | Producción Agraria | es_ES |
dc.contributor.department | Nekazaritza Ekoizpena | eu |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.identifier.doi | 10.1128/aem.69.11.6644-6649.2003 | |
dc.relation.publisherversion | https://doi.org/10.1128/aem.69.11.6644-6649.2003 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |