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dc.creatorFernández San Millán, Aliciaes_ES
dc.creatorMartín Ortigosa, Susanaes_ES
dc.creatorHervás Stubbs, Sandraes_ES
dc.creatorCorral-Martínez, Patriciaes_ES
dc.creatorSeguí-Simarro, José M.es_ES
dc.creatorGaétan, Julienes_ES
dc.creatorCoursaget, Pierrees_ES
dc.creatorVeramendi Charola, Jones_ES
dc.date.accessioned2019-01-16T08:07:58Z
dc.date.available2019-01-16T08:07:58Z
dc.date.issued2008
dc.identifier.issn1467-7644 (Print)
dc.identifier.issn1467-7652 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31982
dc.description.abstractCervical cancer is the second most prevalent cancer in women worldwide. It is linked to infection with human papillomavirus (HPV). As the virus cannot be propagated in culture, vaccines based on virus‐like particles have been developed and recently marketed. However, their high costs constitute an important drawback for widespread use in developing countries, where the incidence of cervical cancer is highest. In a search for alternative production systems, the major structural protein of the HPV‐16 capsid, L1, was expressed in tobacco chloroplasts. A very high yield of production was achieved in mature plants (~3 mg L1/g fresh weight; equivalent to 24% of total soluble protein). This is the highest expression level of HPV L1 protein reported in plants. A single mature plant synthesized ~240 mg of L1. The chloroplast‐derived L1 protein displayed conformation‐specific epitopes and assembled into virus‐like particles, visible by transmission electron microscopy. Furthermore, leaf protein extracts from L1 transgenic plants were highly immunogenic in mice after intraperitoneal injection, and neutralizing antibodies were detected. Taken together, these results predict a promising future for the development of a plant‐based vaccine against HPV.en
dc.description.sponsorshipThis work was supported by Grants BIO2005‐00155 and CPE03‐022‐C5‐2 from the Ministerio de Educación y Ciencia and INIA (Spain).en
dc.format.extent15 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherWileyen
dc.relation.ispartofPlant Biotechnology Journal (2008) 6, pp. 427– 441en
dc.rights© 2008 The Authorsen
dc.subjectHuman papillomavirusen
dc.subjectPlant vaccineen
dc.subjectPlastid transformationen
dc.subjectTobaccoen
dc.subjectVirus-like particlesen
dc.titleHuman papillomavirus L1 protein expressed in tobacco chloroplasts self-assembles into virus-like particles that are highly immunogenicen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Instituto de Agrobiotecnologíaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutuaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1111/j.1467-7652.2008.00338.x
dc.relation.publisherversionhttps://doi.org/10.1111/j.1467-7652.2008.00338.x
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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