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dc.creatorPérez, Astrides_ES
dc.creatorCanle, Deliaes_ES
dc.creatorLatasa Osta, Cristinaes_ES
dc.creatorPoza, Margaritaes_ES
dc.creatorBeceiro, Alejandroes_ES
dc.creatorTomás, María del Mares_ES
dc.creatorFernández, Anaes_ES
dc.creatorMallo, Susanaes_ES
dc.creatorPérez, Soniaes_ES
dc.creatorMolina, Franciscaes_ES
dc.creatorVillanueva González, Rosaes_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.creatorBou, Germánes_ES
dc.identifier.issn0066-4804 (Print)
dc.identifier.issn1098-6596 (Electronic)
dc.description.abstractEnterobacter cloacae is an emerging clinical pathogen that may be responsible for nosocomial infections. Management of these infections is often difficult, owing to the high frequency of strains that are resistant to disinfectants and antimicrobial agents in the clinical setting. Multidrug efflux pumps, especially those belonging to the resistance-nodulation-division family, play a major role as a mechanism of antimicrobial resistance in gram-negative pathogens. In the present study, we cloned and sequenced the genes encoding an AcrAcB-TolC-like efflux pump from an E. cloacae clinical isolate (isolate EcDC64) showing a broad antibiotic resistance profile. Sequence analysis showed that the acrR, acrA, acrB, and tolC genes encode proteins that display 79.8%, 84%, 88%, and 82% amino acid identities with the respective homologues of Enterobacter aerogenes and are arranged in a similar pattern. Deletion of the acrA gene to yield an AcrA-deficient EcDC64 mutant (EcΔacrA) showed the involvement of AcrAB-TolC in multidrug resistance in E. cloacae. However, experiments with an efflux pump inhibitor suggested that additional efflux systems also play a role in antibiotic resistance. Investigation of several unrelated isolates of E. cloacae by PCR analysis revealed that the AcrAB system is apparently ubiquitous in this species.en
dc.description.sponsorshipThe study was partly financed by the Consellería de Innovación, Industria y Comercio, Xunta de Galicia (PGIDIT04BTF916028PR), Fondo de Investigaciones Sanitarias (PI040514 and PI061368), and also supported by Ministerio de Sanidad y Consumo, ISCIII, Spanish Network for the Research in Infectious Diseases (REIPI RD06/0008).en
dc.format.extent7 p.
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofAntimicrobial Agents and Chemotherapy, vol. 51, nº. 9, sept. 2007, p. 3247–325en
dc.rights© 2007 American Society for Microbiologyen
dc.subjectEnterobacter cloacaeen
dc.subjectAntibiotic resistanceen
dc.subjectClinical isolateen
dc.titleCloning, nucleotide sequencing, and analysis of the AcrAB-TolC efflux pump of enterobacter cloacae and determination of its involvement in antibiotic resistance in a clinical isolateen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.type.versionVersión publicada / Argitaratu den bertsioaes

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