• Login
    View Item 
    •   Academica-e
    • Departamentos y Centros - Sailak eta Ikastegiak
    • IdAB / Instituto de Agrobiotecnología - Agrobioteknologiako Institutua
    • Artículos de revista IdAB - IdAB Aldizkari artikuluak
    • View Item
    •   Academica-e
    • Departamentos y Centros - Sailak eta Ikastegiak
    • IdAB / Instituto de Agrobiotecnología - Agrobioteknologiako Institutua
    • Artículos de revista IdAB - IdAB Aldizkari artikuluak
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Effect of transcriptional activators SoxS, RobA, and RamA on expression of multidrug efflux pump AcrAB-TolC in enterobacter cloacae

    Thumbnail
    View/Open
    23_Latasa_Effect_of_transcriptional.pdf (671.9Kb)
    Date
    2012
    Author
    Pérez, Astrid 
    Poza, Margarita 
    Aranda, Jesús 
    Latasa Osta, Cristina Upna
    Medrano, Francisco Javier 
    Tomás, María del Mar 
    Romero, Antonio 
    Lasa Uzcudun, Íñigo Upna
    Bou, Germán 
    Version
    Acceso abierto / Sarbide irekia
    xmlui.dri2xhtml.METS-1.0.item-type
    Artículo / Artikulua
    Version
    Versión publicada / Argitaratu den bertsioa
    Impact
     
     
     
    10.1128/aac.01085-12
     
     
     
    Show full item record
    Abstract
    Control of membrane permeability is a key step in regulating the intracellular concentration of antibiotics. Efflux pumps confer innate resistance to a wide range of toxic compounds such as antibiotics, dyes, detergents, and disinfectants in members of the Enterobacteriaceae. The AcrAB-TolC efflux pump is involved in multidrug resistance in Enterobacter cloacae. However, the underlying mechanism ... [++]
    Control of membrane permeability is a key step in regulating the intracellular concentration of antibiotics. Efflux pumps confer innate resistance to a wide range of toxic compounds such as antibiotics, dyes, detergents, and disinfectants in members of the Enterobacteriaceae. The AcrAB-TolC efflux pump is involved in multidrug resistance in Enterobacter cloacae. However, the underlying mechanism that regulates the system in this microorganism remains unknown. In Escherichia coli, the transcription of acrAB is upregulated under global stress conditions by proteins such as MarA, SoxS, and Rob. In the present study, two clinical isolates of E. cloacae, EcDC64 (a multidrug-resistant strain overexpressing the AcrAB-TolC efflux pump) and Jc194 (a strain with a basal AcrAB-TolC expression level), were used to determine whether similar global stress responses operate in E. cloacae and also to establish the molecular mechanisms underlying this response. A decrease in susceptibility to erythromycin, tetracycline, telithromycin, ciprofloxacin, and chloramphenicol was observed in clinical isolate Jc194 and, to a lesser extent in EcDC64, in the presence of salicylate, decanoate, tetracycline, and paraquat. Increased expression of the acrAB promoter in the presence of the above-described conditions was observed by flow cytometry and reverse transcription-PCR, by using a reporter fusion protein (green fluorescent protein). The expression level of the AcrAB promoter decreased in E. cloacae EcDC64 derivates deficient in SoxS, RobA, and RamA. Accordingly, the expression level of the AcrAB promoter was higher in E. cloacae Jc194 strains overproducing SoxS, RobA, and RamA. Overall, the data showed that SoxS, RobA, and RamA regulators were associated with the upregulation of acrAB, thus conferring antimicrobial resistance as well as a stress response in E. cloacae. In summary, the regulatory proteins SoxS, RobA, and RamA were cloned and sequenced for the first time in this species. The involvement of these proteins in conferring antimicrobial resistance through upregulation of acrAB was demonstrated in E. cloacae. [--]
    Subject
    Transcriptional activators, SoxS, RobA, RamA, Expression of multidrug efflux pump AcrAB-TolC, Enterobacter cloacae
     
    Publisher
    American Society for Microbiology
    Published in
    Antimicrobial Agents and Chemotherapy, December 2012 Volume 56 Number 12, p. 6256-6266
    Departament
    IdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
    Publisher version
    https://doi.org/10.1128/aac.01085-12
    URI
    https://hdl.handle.net/2454/32008
    Sponsorship
    This study was supported by Ayudas a la Movilidad (SEIMC), the Fondo de Investigaciones Sanitarias (PI081368, PS09/00687), and SERGAS (PS07/90) and a grant from the Xunta de Galicia (07CSA050916PR) to G.B. A.P. received scholarships from REIPI (Spanish Network for Research in Infectious Diseases).
    Appears in Collections
    • Artículos de revista IdAB - IdAB Aldizkari artikuluak [159]
    Items in Academica-e are protected by copyright with all rights reserved, unless otherwise noted.

     © Universidad Pública de Navarra - Nafarroako Unibertsitate Publikoa
         Repositorio basado en DSpace

    Contact Us | Send Feedback
     

     

    Browse

    All of Academica-eCommunities & CollectionsAuthorsAuthors By Issue DateTitlesSubjectsBy DegreeThis CollectionAuthorsAuthors By Issue DateTitlesSubjectsBy Degree

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

     © Universidad Pública de Navarra - Nafarroako Unibertsitate Publikoa
         Repositorio basado en DSpace

    Contact Us | Send Feedback