Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system
Fecha
2005Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1128/jb.187.15.5318-5329.2005
Resumen
The biofilm formation capacity of Staphylococcus aureus clinical isolates is considered an important virulence
factor for the establishment of chronic infections. Environmental conditions affect the biofilm formation
capacity of S. aureus, indicating the existence of positive and negative regulators of the process. The majority
of the screening procedures for identifying genes involved in biofilm ...
[++]
The biofilm formation capacity of Staphylococcus aureus clinical isolates is considered an important virulence
factor for the establishment of chronic infections. Environmental conditions affect the biofilm formation
capacity of S. aureus, indicating the existence of positive and negative regulators of the process. The majority
of the screening procedures for identifying genes involved in biofilm development have been focused on genes
whose presence is essential for the process. In this report, we have used random transposon mutagenesis and
systematic disruption of all S. aureus two-component systems to identify negative regulators of S. aureus biofilm
development in a chemically defined medium (Hussain-Hastings-White modified medium [HHWm]). The
results of both approaches coincided in that they identified arlRS as a repressor of biofilm development under
both steady-state and flow conditions. The arlRS mutant exhibited an increased initial attachment as well as
increased accumulation of poly-N-acetylglucosamine (PNAG). However, the biofilm formation of the arlRS
mutant was not affected when the icaADBC operon was deleted, indicating that PNAG is not an essential
compound of the biofilm matrix produced in HHWm. Disruption of the major autolysin gene, atl, did not
produce any effect on the biofilm phenotype of an arlRS mutant. Epistatic experiments with global regulators
involved in staphylococcal-biofilm formation indicated that sarA deletion abolished, whereas agr deletion
reinforced, the biofilm development promoted by the arlRS mutation. [--]
Materias
Staphylococcus aureus,
arlRS,
Biofilm development
Editor
American Society for Microbiology
Publicado en
Journal of Bacteriology, Aug. 2005, Vol. 187, No. 15, p. 5318–5329
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This work was supported by the BIO2002-04542-C02
and GEN2003-20234-C06-05 grants from the Comisión Interministerial
de Ciencia y Tecnología and grant 17/2004 from the Departamento
de Educación y Cultura del Gobierno de Navarra, Spain.