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dc.creatorClavijo Palacios, Gabrieles_ES
dc.creatorWilliams, Trevores_ES
dc.creatorSimón de Goñi, Oihanees_ES
dc.creatorMuñoz, Deliaes_ES
dc.creatorCerutti, Martinees_ES
dc.creatorLópez Ferber, Migueles_ES
dc.creatorCaballero Murillo, Primitivoes_ES
dc.date.accessioned2019-01-21T13:24:48Z
dc.date.available2019-01-21T13:24:48Z
dc.date.issued2009
dc.identifier.issn0022-538X (Print)
dc.identifier.issn1098-5514 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/32036
dc.description.abstractThe insecticidal potency of a nucleopolyhedrovirus population (SfNIC) that infects Spodoptera frugiperda (Lepidoptera) is greater than the potency of any of the component genotypes alone. Occlusion bodies (OBs) produced in mixed infections comprising the complete genotype and a deletion genotype are as pathogenic as the natural population of genotypes from the field. To test whether this increased potency was due to the deletion or to some other characteristic of the deletion variant genome, we used the SfNIC-B genome to construct a recombinant virus (SfNIC-BΔ16K) with the same 16.4-kb deletion as that observed in SfNIC-C and another recombinant (SfNIC-BΔpifs) with a deletion encompassing two adjacent genes (pif1 and pif2) that are essential for transmission per os. Mixtures comprising SfNIC-B and SfNIC-B 16K in OB ratios that varied between 10:90 and 90:10 were injected into insects, and the progeny OBs were fed to larvae in an insecticidal potency assay. A densitometric analysis of PCR products indicated that SfNIC-B was generally more abundant than expected in mixtures based on the proportions of OBs used to produce the inocula. Mixtures derived from OB ratios of 10, 25, or 50% of SfNIC-BΔ16K and the corresponding SfNIC-B proportions showed a significant increase in potency compared to SfNIC-B alone. The results of potency assays with mixtures comprising various proportions of SfNIC-B plus SfNIC-BΔpifs were almost identical to the results observed with SfNICB 16K, indicating that deletion of the pif gene region was responsible for the increased potency observed in mixtures of SfNIC-B and each deletion recombinant virus. Subsequently, mixtures produced from OB ratios involving 10 or 90% of SfNIC-BΔ16K with the corresponding proportions of SfNIC-B were subjected to four rounds of per os transmission in larvae. The composition of each experimental mixture rapidly converged to a common equilibrium with a genotypic composition of ~85% SfNIC-B plus 15% SfNIC-BΔ16K. Nearly identical results were observed in peroral-passage experiments involving mixtures of SfNIC-B plus SfNICBΔpifs. We conclude that (i) the deletion of the pif1 and pif2 region is necessary and sufficient to explain the increased potency observed in mixtures of complete and deletion genotypes and (ii) viral populations with decreased ratios of pif1- and pif2-deficient genotypes in the virus population increase the potency of genotypic mixtures and are likely to positively influence the transmission of this pathogen.en
dc.description.sponsorshipThis study was funded by MEC project number AGL2005-07909- CO3-01.en
dc.format.extent10 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Virology, May 2009, Vol. 83, No. 10, p. 5127–5136en
dc.rights© 2009, American Society for Microbiology. All Rights Reserved.en
dc.subjectNucleopolyhedrovirusen
dc.subjectOcclusion bodiesen
dc.subjectSfNIC-BΔ16Ken
dc.subjectSfNIC-BΔpifsen
dc.titleMixtures of complete and pif1- and pif2-deficient genotypes are required for increased potency of an insect nucleopolyhedrovirusen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Instituto de Agrobiotecnologíaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutuaeu
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/jvi.02020-08
dc.relation.publisherversionhttps://doi.org/10.1128/jvi.02020-08
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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