Near-infrared fluorescence imaging as an alternative to bioluminescent bacteria to monitor biomaterial-associated infections
Fecha
2014Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión aceptada / Onetsi den bertsioa
Impacto
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10.1016/j.actbio.2014.03.005
Resumen
Biomaterial-associated infection is one of the most common complications related with the
implantation of any biomedical device. Several in vivo imaging platforms have emerged as
powerful diagnostic tools to longitudinally monitor biomaterial-associated infections in small
animal models. In this study, we directly compared two imaging approaches: bacteria engineered
to produce luciferase to g ...
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Biomaterial-associated infection is one of the most common complications related with the
implantation of any biomedical device. Several in vivo imaging platforms have emerged as
powerful diagnostic tools to longitudinally monitor biomaterial-associated infections in small
animal models. In this study, we directly compared two imaging approaches: bacteria engineered
to produce luciferase to generate bioluminescence and reactive oxygen species (ROS) imaging of
the inflammatory response associated with the infected implant. We performed longitudinal
imaging of bioluminescence associated with bacteria strains expressing plasmid-integrated
luciferase driven by different promoters or a strain with the luciferase gene integrated into the
chromosome. These luminescent strains provided adequate signal for acute (0–4 days) monitoring
of the infection, but the bioluminescence signal decreased over time and leveled off by 7 days
post-implantation. This loss in bioluminescence signal was attributed to changes in the metabolic
activity of the bacteria. In contrast, near-infrared fluorescence imaging of ROS associated with
inflammation to the implant provided sensitive and dose-dependent signals of biomaterialassociated
bacteria. ROS imaging exhibited higher sensitivity than the bioluminescence imaging
and was independent of the bacteria strain. Near-infrared fluorescence imaging of inflammatory
responses represents a powerful alternative to bioluminescence imaging for monitoring
biomaterial-associated bacterial infections. [--]
Materias
Biomaterial-associated infection,
Bioluminescence,
Near infrared fluorescence,
Noninvasive monitoring,
Staphylococcus aureus
Editor
Elsevier
Publicado en
Acta Biomaterialia 10 (2014) 2935-2944
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This work was supported by the Ministerio of Economía y Competitividad (BIO2010-21049, 201120E092), the U.S.A. National Institutes of Health grant R21 AI094624 (A.J.G.), the Georgia Tech/Emory Center for the Engineering of Living Tissues, the Atlanta Clinical and
Translational Science Institute under PHS Grant UL RR025008 from the Clinical and Translational Science Award Program.