Arabidopsis responds to Alternaria alternata volatiles by triggering pPG-independent mechanisms
Fecha
2016Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión aceptada / Onetsi den bertsioa
Impacto
|
10.1104/pp.16.00945
Resumen
Volatile compounds (VCs) emitted by phylogenetically diverse microorganisms (including plant pathogens and microbes that do
not normally interact mutualistically with plants) promote photosynthesis, growth, and the accumulation of high levels of starch in
leaves through cytokinin (CK)-regulated processes. In Arabidopsis (Arabidopsis thaliana) plants not exposed to VCs, plastidic
phosphoglucose ...
[++]
Volatile compounds (VCs) emitted by phylogenetically diverse microorganisms (including plant pathogens and microbes that do
not normally interact mutualistically with plants) promote photosynthesis, growth, and the accumulation of high levels of starch in
leaves through cytokinin (CK)-regulated processes. In Arabidopsis (Arabidopsis thaliana) plants not exposed to VCs, plastidic
phosphoglucose isomerase (pPGI) acts as an important determinant of photosynthesis and growth, likely as a consequence of
its involvement in the synthesis of plastidic CKs in roots. Moreover, this enzyme plays an important role in connecting the Calvin-
Benson cycle with the starch biosynthetic pathway in leaves. To elucidate the mechanisms involved in the responses of plants to
microbial VCs and to investigate the extent of pPGI involvement, we characterized pPGI-null pgi1-2 Arabidopsis plants cultured in
the presence or absence of VCs emitted by Alternaria alternata. We found that volatile emissions from this fungal phytopathogen
promote growth, photosynthesis, and the accumulation of plastidic CKs in pgi1-2 leaves. Notably, the mesophyll cells of pgi1-2
leaves accumulated exceptionally high levels of starch following VC exposure. Proteomic analyses revealed that VCs promote
global changes in the expression of proteins involved in photosynthesis, starch metabolism, and growth that can account for the
observed responses in pgi1-2 plants. The overall data show that Arabidopsis plants can respond to VCs emitted by phytopathogenic
microorganisms by triggering pPGI-independent mechanisms. [--]
Materias
Arabidopsis,
Alternaria alternata,
Volatile compounds,
Cytokinins,
Phosphoglucose isomerase
Editor
American Society of Plant Biologists
Publicado en
Plant Physiology 172(3): 1989-2001 (2016)
Notas
Incluye material complementario
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This work was supported by the Comisión Interministerial de
Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional,
Spain (grant nos. BIO2010–18239 and BIO2013–49125–C2–1–P), by
the Government of Navarra (grant no. IIM010491.RI1), by the
I-Link0939 project from the Ministerio de Economía y Competitividad,
by the Ministry of Education, Youth, and Sports of the Czech
Republic (grant no. LO1204 from the National Program of Sustainability),
by Palacky University institutional support, by predoctoral
fellowships from the Spanish Ministry of Science and Innovation (to
A.M.S.-L. and P.G.-G.), and by postdoctoral fellowships from the
Public University of Navarra (to M.B. and G.A.).