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dc.creatorBorgognone, Alessandraes_ES
dc.creatorCastanera Andrés, Raúles_ES
dc.creatorMorselli, Marcoes_ES
dc.creatorLópez Varas, Leticiaes_ES
dc.creatorRubbi, Liudmillaes_ES
dc.creatorPisabarro de Lucas, Gerardoes_ES
dc.creatorPellegrini, Matteoes_ES
dc.creatorRamírez Nasto, Lucíaes_ES
dc.date.accessioned2019-07-01T09:27:41Z
dc.date.available2019-07-01T09:27:41Z
dc.date.issued2018
dc.identifier.issn1756-1663
dc.identifier.urihttps://hdl.handle.net/2454/33534
dc.description.abstractTransposable elements constitute an important fraction of eukaryotic genomes. Given their mutagenic potential, host-genomes have evolved epigenetic defense mechanisms to limit their expansion. In fungi, epigenetic modifications have been widely studied in ascomycetes, although we lack a global picture of the epigenetic landscape in basidiomycetes. In this study, we analysed the genome-wide epigenetic and transcriptional patterns of the white-rot basidiomycete Pleurotus ostreatus throughout its life cycle. Our results performed by using high-throughput sequencing analyses revealed that strain-specific DNA methylation profiles are primarily involved in the repression of transposon activity and suggest that 21 nt small RNAs play a key role in transposon silencing. Furthermore, we provide evidence that transposon-associated DNA methylation, but not sRNA production, is directly involved in the silencing of genes surrounded by transposons. Remarkably, we found that nucleus-specific methylation levels varied in dikaryotic strains sharing identical genetic complement but different subculture conditions. Finally, we identified key genes activated in the fruiting process through the comparative analysis of transcriptomes. This study provides an integrated picture of epigenetic defense mechanisms leading to the transcriptional silencing of transposons and surrounding genes in basidiomycetes. Moreover, our findings suggest that transcriptional but not methylation reprogramming triggers fruitbody development in P. ostreatus.en
dc.description.sponsorshipThis work was supported by Spanish National Research Plan (Project AGL2014-55971 R) and FEDER funds; Public University of Navarre. AB and LLV hold a PhD scholarship from the Public University of Navarre, RC holds a FPI-PhD scholarship from the Ministry of Economy and Competitiveness and M.M. holds a Philip Whitcome and Dissertation Year Fellowship. M.P. and M.M. were supported by a cooperative agreement with the US Department of Energy Office of Science, Office of Biological and Environmental Research programme under Award DE-FC02-02ER63421.en
dc.format.extent14 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherOxford University Pressen
dc.relation.ispartofDNA Research, 2018, 25(5), 451–464en
dc.rights© The Author(s) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectEpigeneticsen
dc.subjectDNA methylationen
dc.subjectTranscriptionen
dc.subjectLife cycleen
dc.subjectFungien
dc.titleTransposon-associated epigenetic silencing during Pleurotus ostreatus life cycleen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentProducción Agrariaes_ES
dc.contributor.departmentNekazaritza Ekoizpenaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1093/dnares/dsy016
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2014-55971-R/ES/en
dc.relation.publisherversionhttps://doi.org/10.1093/dnares/dsy016
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes


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© The Author(s) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License,
which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact
journals.permissions@oup.com
La licencia del ítem se describe como © The Author(s) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

El Repositorio ha recibido la ayuda de la Fundación Española para la Ciencia y la Tecnología para la realización de actividades en el ámbito del fomento de la investigación científica de excelencia, en la Línea 2. Repositorios institucionales (convocatoria 2020-2021).
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