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dc.creatorGil Puig, Carmenes_ES
dc.creatorLatasa Osta, Cristinaes_ES
dc.creatorGarcía Ona, Enriquees_ES
dc.creatorLázaro, Isidroes_ES
dc.creatorLabairu, Javieres_ES
dc.creatorEcheverz Sarasúa, Maitees_ES
dc.creatorBurgui Erice, Saioaes_ES
dc.creatorGarcía Martínez, Begoñaes_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.creatorSolano Goñi, Cristinaes_ES
dc.date.accessioned2020-05-12T11:15:22Z
dc.date.available2020-05-12T11:15:22Z
dc.date.issued2020
dc.identifier.issn1297-9716
dc.identifier.urihttps://hdl.handle.net/2454/36857
dc.description.abstractSalmonellosis is the second most common food-borne zoonosis in the European Union, with pigs being a major reservoir of this pathogen. Salmonella control in pig production requires multiple measures amongst which vaccination may be used to reduce subclinical carriage and shedding of prevalent serovars, such as Salmonella enterica serovar Typhimurium. Live attenuated vaccine strains offer advantages in terms of enhancing cell mediated immunity and allowing inoculation by the oral route. However, main failures of these vaccines are the limited cross-protection achieved against heterologous serovars and interference with serological monitoring for infection. We have recently shown that an attenuated S. Enteritidis strain (ΔXIII) is protective against S. Typhimurium in a murine infection model. ΔXIII strain harbours 13 chromosomal deletions that make it unable to produce the sigma factor RpoS and synthesize cyclic-di-GMP (c-di-GMP). In this study, our objectives were to test the protective effects of ΔXIII strain in swine and to investigate if the use of ΔXIII permits the discrimination of vaccinated from infected pigs. Results show that oral vaccination of pre-weaned piglets with ΔXIII cross-protected against a challenge with S. Typhimurium by reducing faecal shedding and ileocaecal lymph nodes colonization, both at the time of weaning and slaughter. Vaccinated pigs showed neither faecal shedding nor tissue persistence of the vaccine strain at weaning, ensuring the absence of ΔXIII strain by the time of slaughter. Moreover, lack of the SEN4316 protein in ΔXIII strain allowed the development of a serological test that enabled the differentiation of infected from vaccinated animals (DIVA).en
dc.description.sponsorshipSB was supported by a predoctoral contract from the Public University of Navarra. CG, ME and BG were recipients of postdoctoral contracts under Grant BIO2014-53530-R. This research was supported by grant IIM 13329.RI1 from the Departamento de Innovación, Empresa y Empleo, Government of Navarra and grants BIO2014-53530-R and BIO2017-83035-R from the Spanish Ministry of Economy and Competitiveness (Agencia Española de Investigación/Fondo Europeo de Desarrollo Regional, European Union).en
dc.format.extent10 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherBioMed Centralen
dc.relation.ispartofVeterinary Research, 2020, 51, 3en
dc.rights© The Author(s). 2020 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSalmonellosisen
dc.subjectPigsen
dc.subjectVaccinesen
dc.titleA DIVA vaccine strain lacking RpoS and the secondary messenger c-di-GMP for protection against salmonellosis in pigsen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1186/s13567-019-0730-3
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BIO2014-53530-R/ES/en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83035-R/ES/en
dc.relation.publisherversionhttps://doi.org/10.1186/s13567-019-0730-3
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua, IIM 13329.RI1es


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© The Author(s). 2020 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0
International License, which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as © The Author(s). 2020 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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