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dc.creatorAncín Rípodas, Maríaes_ES
dc.creatorLarraya Reta, Luis Maríaes_ES
dc.creatorFernández San Millán, Aliciaes_ES
dc.creatorVeramendi Charola, Jones_ES
dc.creatorBurch Smith, Tessaes_ES
dc.creatorFarrán Blanch, Inmaculadaes_ES
dc.date.accessioned2020-05-19T06:57:55Z
dc.date.available2020-05-19T06:57:55Z
dc.date.issued2019
dc.identifier.issn2223-7747
dc.identifier.urihttps://hdl.handle.net/2454/36915
dc.description.abstractThioredoxin (Trx) f and NADPH-dependent Trx reductase C (NTRC) have both been proposed as major redox regulators of starch metabolism in chloroplasts. However, little is known regarding the specific role of each protein in this complex mechanism. To shed light on this point, tobacco plants that were genetically engineered to overexpress the NTRC protein from the chloroplast genome were obtained and compared to previously generated Trx f-overexpressing transplastomic plants. Likewise, we investigated the impact of NTRC and Trx f deficiency on starch metabolism by generating Nicotiana benthamiana plants that were silenced for each gene. Our results demonstrated that NTRC overexpression induced enhanced starch accumulation in tobacco leaves, as occurred with Trx f. However, only Trx f silencing leads to a significant decrease in the leaf starch content. Quantitative analysis of enzyme activities related to starch synthesis and degradation were determined in all of the genotypes. Zymographic analyses were additionally performed to compare the amylolytic enzyme profiles of both transplastomic tobacco plants. Our findings indicated that NTRC overexpression promotes the accumulation of transitory leaf starch as a consequence of a diminished starch turnover during the dark period, which seems to be related to a significant reductive activation of ADP-glucose pyrophosphorylase and/or a deactivation of a putative debranching enzyme. On the other hand, increased starch content in Trx f-overexpressing plants was connected to an increase in the capacity of soluble starch synthases during the light period. Taken together, these results suggest that NTRC and the ferredoxin/Trx system play distinct roles in starch turnover.en
dc.description.sponsorshipThis research was funded by the by the Spanish Ministry of Science and Innovation, grants number AGL2010-15107 and AGL2016-79868. M.A. was supported by FPU pre-doctoral fellowship from Spanish Ministry of Education, Culture and Sports. T.B.-S. was supported by NSF 1456761.en
dc.format.extent20 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofPlants, 2019, 8 (12), 543en
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY)en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectStarch metabolismen
dc.subjectThioredoxinen
dc.subjectNTRCen
dc.subjectRedox regulationen
dc.subjectChloroplasten
dc.subjectVIGSen
dc.titleNTRC and thioredoxin f overexpression differentially induces starch accumulation in tobacco leavesen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.3390/plants8120543
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/AGL2016-79868en
dc.relation.publisherversionhttps://doi.org/10.3390/plants8120543
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY)
Except where otherwise noted, this item's license is described as © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY)

El Repositorio ha recibido la ayuda de la Fundación Española para la Ciencia y la Tecnología para la realización de actividades en el ámbito del fomento de la investigación científica de excelencia, en la Línea 2. Repositorios institucionales (convocatoria 2020-2021).
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