Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue
Date
2016Author
Version
Acceso abierto / Sarbide irekia
Type
Artículo / Artikulua
Version
Versión publicada / Argitaratu den bertsioa
Impact
|
10.1016/j.scr.2015.12.002
Abstract
In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-a ...
[++]
In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation ability to the three germ layers. [--]
Subject
Mesenchymal stem cells,
Adipose tissue,
G15.AO.
Publisher
Elsevier
Published in
Stem Cell Research, 2016, 16(1), 20-23
Departament
Universidad Pública de Navarra. Departamento de Ciencias de la Salud /
Nafarroako Unibertsitate Publikoa. Osasun Zientziak Saila
Publisher version
Sponsorship
This work was supported by the 'Torres Quevedo' Subprogram, Ministry of Economy and Competitiveness (PTQ-11-04777) to JRRM; the Institute of Health Carlos III (PI13/00862) to JRRM, and RETIC (RD12/0019/0031) to FP; and the Fundación Bancaria Caja Navarra (70270) to JRRM.