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dc.creatorRamírez Zapata, Dianaes_ES
dc.creatorRamos, Cayoes_ES
dc.creatorAguilera, Selenees_ES
dc.creatorBardají Goikoetxea, Leirees_ES
dc.creatorMartínez Gil, Martaes_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.date.accessioned2020-10-06T14:46:34Z
dc.date.available2020-10-06T14:46:34Z
dc.date.issued2020
dc.identifier.urihttps://hdl.handle.net/2454/38352
dc.description.abstractThe widely conserved Csr/Rsm (carbon storage regulator/repressor of stationary-phase metabolites) post-transcriptional regulatory system controls diverse phenotypes involved in bacterial pathogenicity and virulence. Here we show that Pseudomonas amygdali pv. phaseolicola 1448A contains seven rsm genes, four of which are chromosomal. In RNAseq analyses, only rsmE was thermoregulated, with increased expression at 18 °C, whereas the antagonistic sRNAs rsmX1, rsmX4, rsmX5 and rsmZ showed increased levels at 28 °C. Only double rsmA-rsmE mutants showed significantly altered phenotypes in functional analyses, being impaired for symptom elicitation in bean, including in planta growth, and for induction of the hypersensitive response in tobacco. Double mutants were also non-motile and were compromised for the utilization of different carbon sources. These phenotypes were accompanied by reduced mRNA levels of the type III secretion system regulatory genes hrpL and hrpA, and the flagellin gene, fliC. Biosynthesis of the phytotoxin phaseolotoxin by mutants in rsmA and rsmE was delayed, occurring only in older cultures, indicating that these rsm homologues act as inductors of toxin synthesis. Therefore, genes rsmA and rsmE act redundantly, although with a degree of specialization, to positively regulate diverse phenotypes involved in niche colonization. Additionally, our results suggest the existence of a regulatory molecule different from the Rsm proteins and dependent on the GacS/GacA (global activator of antibiotic and cyanide production) system, which causes the repression of phaseolotoxin biosynthesis at high temperatures.en
dc.description.sponsorshipThis work was funded by the Spanish Plan Nacional I+D+I grants AGL2017-82492-C2-1-R and AGL2017-82492-C2-2-R, from the Ministerio de Economía y Competitividad (MINECO), co-financed by the Fondo Europeo de Desarrollo Regional (FEDER). DR-Z was supported by a Formación de Personal Investigador (FPI) contract (reference BES-2015-074315, Ministerio de Economía y Competitividad, Spain).en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofMicroorganisms 2020, 8, 1536en
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPhaseolotoxinen
dc.subjectPhytotoxinsen
dc.subjectGacS/GacA systemen
dc.subjectTwo-component signal transduction systemen
dc.subjectSmall regulatory RNAsen
dc.subjectPost-transcriptional regulationen
dc.subjectVirulenceen
dc.subjectPseudomonas syringaeen
dc.subjectPseudomonas savastanoien
dc.titleTwo homologues of the global regulator Csr/Rsm redundantly control phaseolotoxin biosynthesis and virulence in the plant pathogen Pseudomonas amygdali pv. phaseolicola 1448Aen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.3390/microorganisms8101536
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82492-C2-2-R/ES/en
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms8101536
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license.
Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.

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