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Antibiofilm activity of flavonoids on staphylococcal biofilms through targeting BAP amyloids
dc.creator | Matilla Cuenca, Leticia | es_ES |
dc.creator | Gil Puig, Carmen | es_ES |
dc.creator | Cuesta Ferre, Sergio | es_ES |
dc.creator | Rapún Araiz, Beatriz | es_ES |
dc.creator | Mira, Alex | es_ES |
dc.creator | Lasa Uzcudun, Íñigo | es_ES |
dc.creator | Valle Turrillas, Jaione | es_ES |
dc.creator | Ziemité, Miglé | es_ES |
dc.date.accessioned | 2021-06-16T08:48:59Z | |
dc.date.available | 2021-06-16T08:48:59Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://hdl.handle.net/2454/39942 | |
dc.description | Incluye material complementario | es_ES |
dc.description.abstract | The opportunistic pathogen Staphylococcus aureus is responsible for causing infections related to indwelling medical devices, where this pathogen is able to attach and form biofilms. The intrinsic properties given by the self-produced extracellular biofilm matrix confer high resistance to antibiotics, triggering infections difficult to treat. Therefore, novel antibiofilm strategies targeting matrix components are urgently needed. The biofilm associated protein, Bap, expressed by staphylococcal species adopts functional amyloid-like structures as scaffolds of the biofilm matrix. In this work we have focused on identifying agents targeting Bap-related amyloid-like aggregates as a strategy to combat S. aureus biofilm-related infections. We identified that the flavonoids, quercetin, myricetin and scutellarein specifically inhibited Bap-mediated biofilm formation of S. aureus and other staphylococcal species. By using in vitro aggregation assays and the cell-based methodology for generation of amyloid aggregates based on the Curli-Dependent Amyloid Generator system (C-DAG), we demonstrated that these polyphenols prevented the assembly of Bap-related amyloid-like structures. Finally, using an in vivo catheter infection model, we showed that quercetin and myricetin significantly reduced catheter colonization by S. aureus. These results support the use of polyphenols as anti-amyloids molecules that can be used to treat biofilm-related infections. | en |
dc.description.sponsorship | This research was supported by Grants RTI2018-096011-B-I00 from Ministry of Science and Innovation and PI011 KILL-BACT from the Government of Navarra. | en |
dc.format.extent | 12 p. | |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Nature Research | en |
dc.relation.ispartof | Scientific Reports, (2020) 10:18968 | en |
dc.rights | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Staphylococcus aureus | en |
dc.subject | Biofilms | en |
dc.subject | Flavonoids | en |
dc.subject | Bap (biofilm associated protein) | en |
dc.title | Antibiofilm activity of flavonoids on staphylococcal biofilms through targeting BAP amyloids | en |
dc.type | info:eu-repo/semantics/article | en |
dc.type | Artículo / Artikulua | es |
dc.contributor.department | Ciencias de la Salud | es_ES |
dc.contributor.department | Osasun Zientziak | eu |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.identifier.doi | 10.1038/s41598-020-75929-2 | |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096011-B-I00/ES/ | en |
dc.relation.publisherversion | https://doi.org/10.1038/s41598-020-75929-2 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
dc.contributor.funder | Gobierno de Navarra / Nafarroako Gobernua, PI011 KILL-BACT | es |
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