The Pbo cluster from Pseudomonas syringae pv. phaseolicola NPS3121 is thermoregulated and required for phaseolotoxin biosynthesis
Fecha
2021Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
Impacto
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10.3390/toxins13090628
Resumen
The bean (Phaseolus vulgaris) pathogen Pseudomonas syringae pv. phaseolicola NPS3121 synthe-sizes phaseolotoxin in a thermoregulated way, with optimum production at 18 °C. Gene PSPPH_4550 was previously shown to be thermoregulated and required for phaseolotoxin bio-synthesis. Here, we established that PSPPH_4550 is part of a cluster of 16 genes, the Pbo cluster, included in a genomic island with ...
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The bean (Phaseolus vulgaris) pathogen Pseudomonas syringae pv. phaseolicola NPS3121 synthe-sizes phaseolotoxin in a thermoregulated way, with optimum production at 18 °C. Gene PSPPH_4550 was previously shown to be thermoregulated and required for phaseolotoxin bio-synthesis. Here, we established that PSPPH_4550 is part of a cluster of 16 genes, the Pbo cluster, included in a genomic island with a limited distribution in P. syringae and unrelated to the posses-sion of the phaseolotoxin biosynthesis cluster. We identified typical non-ribosomal peptide syn-thetase, and polyketide synthetase domains in several of the pbo deduced products. RT-PCR and the analysis of polar mutants showed that the Pbo cluster is organized in four transcriptional units, including one monocistronic and three polycistronic. Operons pboA and pboO are both es-sential for phaseolotoxin biosynthesis, while pboK and pboJ only influence the amount of toxin produced. The three polycistronic units were transcribed at high levels at 18 °C but not at 28 °C, whereas gene pboJ was constitutively expressed. Together, our data suggest that the Pbo cluster synthesizes secondary metabolite(s), which could participate in the regulation of phaseolotoxin biosynthesis. [--]
Materias
Pseudomonas syringae,
Pseudomonas amygdali,
Pseudomonas savastanoi,
Phaseolotoxin,
Pbo cluster,
Non-ribosomal peptide synthetases,
Genomic island,
Antimetabolite toxin,
Polyketide synthetase
Editor
MDPI
Publicado en
Toxins 2021, 13, 628
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Multidisciplinary Research in Applied Biology - IMAB
Versión del editor
Entidades Financiadoras
The authors thank the Consejo Nacional de Ciencia y Tecnología (CONACyT) for the
scholarship granted to Lizeth Guardado-Valdivia. The work reported was funded by research grant
CB-2015-01-255155 from the CONACyT, to S. Aguilera, and by the Spanish Plan Nacional I+D+I grant
AGL2017-82492-C2-2-R, from the Ministerio de Economía y Competitividad (MINECO), co-financed
by the Fondo Europeo de Desarrollo Regional (FEDER), to J. Murillo.