Generation of variability in Chrysodeixis includens nucleopolyhedrovirus (ChinNPV): the role of a single variant
Fecha
2021Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
Impacto
|
10.3390/v13101895
Resumen
The mechanisms generating variability in viruses are diverse. Variability allows baculoviruses
to evolve with their host and with changes in their environment. We examined the role of
one genetic variant of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and its contribution
to the variability of the virus under laboratory conditions. A mixture of natural isolates (ChinNPVMex1)
containe ...
[++]
The mechanisms generating variability in viruses are diverse. Variability allows baculoviruses
to evolve with their host and with changes in their environment. We examined the role of
one genetic variant of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and its contribution
to the variability of the virus under laboratory conditions. A mixture of natural isolates (ChinNPVMex1)
contained two genetic variants that dominated over other variants in individual larvae that
consumed high (ChinNPV-K) and low (ChinNPV-E) concentrations of inoculum. Studies on the
ChinNPV-K variant indicated that it was capable of generating novel variation in a concentrationdependent
manner. In cell culture, cells inoculated with high concentrations of ChinNPV-K produced
OBs with the ChinNPV-K REN profile, whereas a high diversity of ChinNPV variants was recovered
following plaque purification of low concentrations of ChinNPV-K virion inoculum. Interestingly,
the ChinNPV-K variant could not be recovered from plaques derived from low concentration inocula
originating from budded virions or occlusion-derived virions of ChinNPV-K. Genome sequencing
revealed marked differences between ChinNPV-K and ChinNPV-E, with high variation in the
ChinNPV-K genome, mostly due to single nucleotide polymorphisms. We conclude that ChinNPV-K
is an unstable genetic variant that is responsible for generating much of the detected variability in
the natural ChinNPV isolates used in this study. [--]
Materias
ChinNPV,
Variability,
Prevalence,
SNPs,
Concentration,
Chrysodeixis includens
Editor
MDPI
Publicado en
Viruses 2021, 13, 1895
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Multidisciplinary Research in Applied Biology - IMAB
Versión del editor
Entidades Financiadoras
This research was funded by the Ministerio de Economía y Competitividad; grant number
AGL2017-83498-C2-1-R. E.A. received an UPNA studentship. I.B. received a Torres-Quevedo grant
and was funded by Bioinsectis SL during the preparation of this manuscript.