Gender-dependent deregulation of linear and circular RNA variants of homer1 in the entorhinal cortex of Alzheimer’s disease
Fecha
2021Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.3390/ijms22179205
Resumen
The HOMER1 gene is involved in synaptic plasticity, learning and memory. Recent studies show that circular RNA derived from HOMER1 (circHOMER1) expression is altered in some Alzheimer’s disease (AD) brain regions. In addition, HOMER1 messenger (mRNA) levels have been associated with β-Amyloid (Aβ) deposits in brain cortical regions. Our aim was to measure the expression levels of HOMER1 circRNAs ...
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The HOMER1 gene is involved in synaptic plasticity, learning and memory. Recent studies show that circular RNA derived from HOMER1 (circHOMER1) expression is altered in some Alzheimer’s disease (AD) brain regions. In addition, HOMER1 messenger (mRNA) levels have been associated with β-Amyloid (Aβ) deposits in brain cortical regions. Our aim was to measure the expression levels of HOMER1 circRNAs and their linear forms in the human AD entorhinal cortex. First, we showed downregulation of HOMER1B/C and HOMER1A mRNA and hsa_circ_0006916 and hsa_circ_0073127 levels in AD female cases compared to controls by RT-qPCR. A positive correlation was observed between HOMER1B/C, HOMER1A mRNA, and hsa_circ_0073128 with HOMER1B/C protein only in females. Global average area of Aβ deposits in entorhinal cortex samples was negatively correlated with HOMER1B/C, HOMER1A mRNA, and hsa_circ_0073127 in both genders. Furthermore, no differences in DNA methylation were found in two regions of HOMER1 promoter between AD cases and controls. To sum up, we demonstrate that linear and circular RNA variants of HOMER1 are downregulated in the entorhinal cortex of female patients with AD. These results add to the notion that HOMER1 and its circular forms could be playing a female-specific role in the pathogenesis of AD. [--]
Materias
Alzheimer's disease,
Entorhinal cortex,
HOMER1,
Sleep deprivation,
circRNA,
mRNA,
Calcium,
Synapses,
Amyloid beta,
Gender,
Female
Editor
MDPI
Publicado en
International Journal of Molecular Sciences, 22 (17), 9205
Departamento
Universidad Pública de Navarra. Departamento de Ciencias de la Salud /
Nafarroako Unibertsitate Publikoa. Osasun Zientziak Saila
Versión del editor
Entidades Financiadoras
This work was supported by the Spanish Government through grants from the Institute of Health Carlos III (FIS PI20/01701). In addition, A.U.-C. received two grants 'Doctorados industriales 2018–2020' and 'Contrato predoctoral en investigación en ciencias y tecnologías de la salud en el periodo 2019–2022', both of them founded by the Government of Navarra. B.A. is supported by a PFIS fellowship from the Spanish Government (FI18/00150) and M.M. received a grant 'Programa de intensificación' (LCF/PR/PR15/51100006) funded by Fundación Bancaria 'la Caixa' and Fundación Caja-Navarra and 'Contrato de intensificación' from the Institute of Health Carlos III (INT19/00029).