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dc.creatorDurán Lázaro, Maríaes_ES
dc.creatorSan Emeterio Garciandía, Leticiaes_ES
dc.creatorCanals Tresserras, Rosa Maríaes_ES
dc.date.accessioned2022-04-07T07:47:43Z
dc.date.available2022-04-07T07:47:43Z
dc.date.issued2021
dc.identifier.issn2079-7737
dc.identifier.urihttps://hdl.handle.net/2454/42673
dc.description.abstractFungal endophytes develop inside plants without visible external signs, and they may confer adaptive advantages to their hosts. Culturing methods have been traditionally used to recognize the fungal endophytic assemblage, but novel metabarcoding techniques are being increasingly applied. This study aims to characterize the fungal endophytic assemblage in shoots, rhizomes and roots of the tall grass Brachypodium rupestre growing in a large area of natural grasslands with a continuum of anthropized disturbance regimes. Seven out of 88 taxa identified via metabarcoding accounted for 81.2% of the reads (Helotiaceae, Lachnum sp. A, Albotricha sp. A, Helotiales A, Agaricales A, Mycena sp. and Mollisiaceae C), revealing a small group of abundant endophytes and a large group of rare species. Although both methods detected the same trends in richness and fungal diversity among the tissues (root > rhizome > shoot) and grasslands (low-diversity >high-diversity grasslands), the metabarcoding tool identified 5.8 times more taxa than the traditional culturing method (15 taxa) but, surprisingly, failed to sequence the most isolated endophyte on plates, Omnidemptus graminis. Since both methods are still subject to important constraints, both are required to obtain a complete characterization of the fungal endophytic assemblage of the plant species.en
dc.description.sponsorshipM.D. got funding through UPNA’s doctorate scholarship. This research was supported by 'la Caixa' Foundation and CAN foundation (LCF/PR/PR13/51080004), Ecoembes-SEO/Birdlife (Libera 2017), Government of Navarra (STEM research project 2018) and Interreg Sudoe Programme, European Regional Development Fund, European-Union, Open2preserve Project (SOE2/P5/E0804).en
dc.format.extent20 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofBiology, 2021, 10, 1246en
dc.rights© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.es_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBrachypodium rupestreen
dc.subjectCulturingen
dc.subjectFireen
dc.subjectGrazingen
dc.subjectMetabarcodingen
dc.subjectMycobiomeen
dc.titleComparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimesen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentInstitute on Innovation and Sustainable Development in Food Chain - ISFOODes_ES
dc.contributor.departmentAgronomía, Biotecnología y Alimentaciónes_ES
dc.contributor.departmentAgronomia, Bioteknologia eta Elikaduraeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.3390/biology10121246
dc.relation.publisherversionhttps://doi.org/10.3390/biology10121246
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua, CENEDUCA18es
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes


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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
Except where otherwise noted, this item's license is described as © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.

El Repositorio ha recibido la ayuda de la Fundación Española para la Ciencia y la Tecnología para la realización de actividades en el ámbito del fomento de la investigación científica de excelencia, en la Línea 2. Repositorios institucionales (convocatoria 2020-2021).
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