Three classes of hemoglobins are required for optimal vegetative and reproductive growth of Lotus japonicus: genetic and biochemical characterization of LjGlb2-1
Fecha
2021Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-85775-R/ES/
AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113985GB-I00/ES/
AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094623-B-C22/ES/
MINECO//BES-2015-073059/ES/
Impacto
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10.1093/jxb/erab376
Resumen
Legumes express two major types of hemoglobins, namely symbiotic (leghemoglobins) and non-symbiotic (phytoglobins), with the latter being categorized into three classes according to phylogeny and biochemistry. Using knockout mutants, we show that all three phytoglobin classes are required for optimal vegetative and reproductive development of Lotus japonicus. The mutants of two class 1 phytoglobi ...
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Legumes express two major types of hemoglobins, namely symbiotic (leghemoglobins) and non-symbiotic (phytoglobins), with the latter being categorized into three classes according to phylogeny and biochemistry. Using knockout mutants, we show that all three phytoglobin classes are required for optimal vegetative and reproductive development of Lotus japonicus. The mutants of two class 1 phytoglobins showed different phenotypes: Ljglb1-1 plants were smaller and had relatively more pods, whereas Ljglb1-2 plants had no distinctive vegetative phenotype and produced relatively fewer pods. Non-nodulated plants lacking LjGlb2-1 showed delayed growth and alterations in the leaf metabolome linked to amino acid processing, fermentative and respiratory pathways, and hormonal balance. The leaves of mutant plants accumulated salicylic acid and contained relatively less methyl jasmonic acid, suggesting crosstalk between LjGlb2-1 and the signaling pathways of both hormones. Based on the expression of LjGlb2-1 in leaves, the alterations of flowering and fruiting of nodulated Ljglb2-1 plants, the developmental and biochemical phenotypes of the mutant fed on ammonium nitrate, and the heme coordination and reactivity of the protein toward nitric oxide, we conclude that LjGlb2-1 is not a leghemoglobin but an unusual class 2 phytoglobin. For comparison, we have also characterized a close relative of LjGlb2-1 in Medicago truncatula, MtLb3, and conclude that this is an atypical leghemoglobin. [--]
Materias
Hemoglobins,
Lotus japonicus,
Medicago truncatula,
Mutants,
Nitric oxide,
Symbiosis
Editor
Oxford University Press
Publicado en
Journal of Experimental Botany, Vol. 72 (22), p. 7778–7791, December 2021,
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Multidisciplinary Research in Applied Biology - IMAB /
Universidad Pública de Navarra. Departamento de Ciencias /
Nafarroako Unibertsitate Publikoa. Zientziak Saila
Versión del editor
Entidades Financiadoras
This work was supported by grants AGL2017-85775-R, PID2020-113985GB-I00, and RTI2018-094623-B-C22 from the Agencia Estatal de Investigación (AEI) and by Gobierno de Aragón (group A09_20R). IV was a ‘Formación de Personal Investigador’ fellow (BES-2015-073059) and EL is a ‘Ramón y Cajal’ fellow (RYC2018-023867-I), both funded by AEI.