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dc.creatorLópez Gómez, Pedroes_ES
dc.creatorSmith, Edward N.es_ES
dc.creatorBota, Pedroes_ES
dc.creatorCornejo Ibergallartu, Alfonsoes_ES
dc.creatorUrra Rodríguez, Marinaes_ES
dc.creatorBuezo Bravo, Javieres_ES
dc.creatorMorán Juez, José Fernandoes_ES
dc.identifier.citationLópez-Gómez, P.; Smith, E.N.; Bota, P.; Cornejo, A.; Urra, M.; Buezo, J.; Moran, J.F.. (2022). Tryptophan Levels as a Marker of Auxins and Nitric Oxide Signaling. Plants. 11,10 1304-1304 .en
dc.description.abstractThe aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA), which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of the most common intermediates. Auxin signaling is well known to interact with free radical nitric oxide (NO) to perform a more complex effect, including the regulation of root organogenesis and nitrogen nutrition. To fathom the link between IAA and NO, we use a metabolomic approach to analyze the contents of low-molecular-mass molecules in cultured cells of Arabidopsis thaliana after the application of S-nitrosoglutathione (GSNO), an NO donor or IAOx. We separated the crude extracts of the plant cells through ion-exchange columns, and subsequent fractions were analyzed by gas chromatography-mass spectrometry (GC-MS), thus identifying 26 compounds. A principal component analysis (PCA) was performed on N-metabolism-related compounds, as classified by the Kyoto Encyclopedia of Genes and Genomes (KEGG). The differences observed between controls and treatments are mainly explained by the differences in Trp contents, which are much higher in controls. Thus, the Trp is a shared response in both auxin- and NO-mediated signaling, evidencing some common signaling mechanism to both GSNO and IAOx. The differences in the low-molecularmass- identified compounds between GSNO- and IAOx-treated cells are mainly explained by their concentrations in benzenepropanoic acid, which is highly associated with IAA levels, and salicylic acid, which is related to glutathione. These results show that the contents in Trp can be a marker for the study of auxin and NO signaling.en
dc.description.sponsorshipThis work was supported by grants from the Spanish Ministry of Science and Innovation (AGL2017-52396-P, co-financed by the European Regional Development Fund, FEDER) and a grant from the Public University of Navarre (Res 309/2022) to J.F.M. M.U. is a recipient of a pre-doctoral fellowship from the Government of Navarre, Spain. J.B. and P.L. have received pre-doctoral fellow- ships from the Public University of Navarre, Spain. J.B. was supported by the Romanian Ministry of Education and research, grant CNCS-UEFISCDI, project number PN-III-P4-ID-PCE-2020-2696, within PNCDI III.en
dc.format.extent18 p.
dc.relation.ispartofPlants, 2022, 11 (10),1304-1304en
dc.rights© 2022 by the authors. Creative Commons Attribution 4.0en
dc.subjectNitric oxideen
dc.subjectPrincipal-component analysisen
dc.titleTryptophan levels as a marker of auxins and nitric oxide signalingen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentInstitute for Advanced Materials and Mathematics - INAMAT2es_ES
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABes_ES
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-52396-Pen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes

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© 2022 by the authors. Creative Commons Attribution 4.0
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El Repositorio ha recibido la ayuda de la Fundación Española para la Ciencia y la Tecnología para la realización de actividades en el ámbito del fomento de la investigación científica de excelencia, en la Línea 2. Repositorios institucionales (convocatoria 2020-2021).
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