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dc.creatorArrizubieta Celaya, Maitees_ES
dc.creatorSimón de Goñi, Oihanees_ES
dc.creatorRicarte Bermejo, Adrianaes_ES
dc.creatorLópez-Ferber, Migueles_ES
dc.creatorWilliams, Trevores_ES
dc.creatorCaballero Murillo, Primitivoes_ES
dc.date.accessioned2022-07-18T10:52:20Z
dc.date.available2022-07-18T10:52:20Z
dc.date.issued2022
dc.identifier.citationArrizubieta-Celaya, M.; Simón-de-Goñi, O.; Ricarte-Bermejo, A.; López-Ferber, M.; Williams, T.; Caballero-Murillo, P. (2022). Coocclusion of Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera multiple nucleopolyhedrovirus (HearMNPV): pathogenicity and stability in homologous and heterologous hosts. Viruses. 14, 4en
dc.identifier.issn1999-4915
dc.identifier.urihttps://hdl.handle.net/2454/43342
dc.description.abstractHelicoverpa armigera single nucleopolyhedrovirus (HearSNPV) is a virulent pathogen of lepidopterans in the genera Heliothis and Helicoverpa, whereas Helicoverpa armigera multiple nu-cleopolyhedrovirus (HearSNPV) is a different virus species with a broader host range. This study aimed to examine the consequences of coocclusion of HearSNPV and HearMNPV on the patho-genicity, stability and host range of mixed-virus occlusion bodies (OBs). HearSNPV OBs were approximately 6-fold more pathogenic than HearMNPV OBs, showed faster killing by approximately 13 h, and were approximately 45% more productive in terms of OB production per larva. For coocclusion, H. armigera larvae were first inoculated with HearMNPV OBs and subsequently inoculated with HearSNPV OBs at intervals of 0-72 h after the initial inoculation. When the interval between inoculations was 12-24 h, OBs collected from virus-killed insects were found to comprise 41¿57% of HearSNPV genomes, but the prevalence of HearSNPV genomes was greatly reduced (3- 4%) at later time points. Quantitative PCR (qPCR) analysis revealed the presence of HearSNPV genomes in a small fraction of multinucleocapsid ODVs representing 0.47¿0.88% of the genomes quan-tified in ODV samples, indicating that both viruses had replicated in coinfected host cells. End-point dilution assays on ODVs from cooccluded mixed-virus OBs confirmed the presence of both viruses in 41.9¿55.6% of wells that were predicted to have been infected by a single ODV. A control exper-iment indicated that this result was unlikely to be due to the adhesion of HearSNPV ODVs to HearMNPV ODVs or accidental contamination during ODV band extraction. Therefore, the dispar-ity between the qPCR and end-point dilution estimates of the prevalence of mixed-virus ODVs likely reflected virus-specific differences in replication efficiency in cell culture and the higher in-fectivity of pseudotyped ODVs that were produced in coinfected parental cells. Bioassays on H. armigera, Spodoptera frugiperda and Mamestra brassicae larvae revealed that mixed-virus OBs were capable of infecting heterologous hosts, but relative potency values largely reflected the proportion of HearMNPV present in each mixed-virus preparation. The cooccluded mixtures were unstable in serial passage; HearSNPV rapidly dominated during passage in H. armigera whereas HearMNPV rapidly dominated during passage in the heterologous hosts. We conclude that mixed-virus coocclusion technology may be useful for producing precise mixtures of viruses with host range properties suitable for the control of complexes of lepidopteran pests in particular crops, although this requires validation by field testing.en
dc.description.sponsorshipThis research was funded by the Ministerio de Economía y Competitividad, Spain, project numbers AGL2014-57752-C2-1-R, AGL2017-83498-C2-1-R, PID2020-117062RB-C21 and Gobierno de Navarra grant numbers IIQ14065.RI1 and IIM14200.RI1. The APC was funded by Ministerio de Economía y Competitividad, Spain, project number PID2020-117062RB-C21. M.A. received a predoctoral scholarship from CSIC, Spain.en
dc.format.extent19 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofViruses, 2022, 14 (4), p. 687en
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) licenseen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBaculoviridaeen
dc.subjectHearNPVen
dc.subjectHost rangeen
dc.subjectMamestra brassicaeen
dc.subjectOcclusion derived virionen
dc.subjectOld world cotton bollwormen
dc.subjectSpodoptera frugiperdaen
dc.subjectVirus insecticideen
dc.titleCoocclusion of Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera multiple nucleopolyhedrovirus (HearMNPV): pathogenicity and stability in homologous and heterologous hostsen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.date.updated2022-07-18T10:52:20Z
dc.contributor.departmentInstitute for Multidisciplinary Research in Applied Biology - IMABes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.3390/v14040687
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2014-57752-C2-1-R/ES/en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83498-C2-1-R/ES/en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117062RB-C21/ES/en
dc.relation.publisherversionhttps://doi.org/10.3390/v14040687
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes


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