Lipid status of A2780 ovarian cancer cells after treatment with ruthenium complex modified with carbon dot nanocarriers: a multimodal SR-FTIR spectroscopy and MALDI TOF Mass Spectrometry Study MALDI TOF Mass Spectrometry Study
Fecha
2022Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.3390/cancers14051182
Resumen
In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(¿5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spe ...
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In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(¿5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane—water interface region. [--]
Materias
Anticancer Ru metallodrug,
Carbon dots,
N-doped carbon dots,
Drug nanocarriers,
Lipids,
MALDI TOF MS,
SR-FTIR spectroscopy
Editor
MDPI
Publicado en
Cancers, 2022, 14 (5),1182-1200
Departamento
Universidad Pública de Navarra. Departamento de Ciencias /
Nafarroako Unibertsitate Publikoa. Zientziak Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Advanced Materials and Mathematics - INAMAT2
Versión del editor
Entidades Financiadoras
ALBA Synchrotron, MIRAS Beamline (experiment No. 2019093770). This work was also supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (grant 451-03-68/2020-14/200017); FCT(CQM Base Fund—UIDB/00674/2020, Programmatic Fund—UIDP/00674/2020); Madeira 14-20 Program (project Reforço do Investimentoem Equipamentos e Infrastructures Científcasna RAM (PROEQUIPRAM) M1420-01-0145-FEDER-000008); and Agência Regional para o Desenvolvimento da Investigação, Tecnologia e Inovação (ARDITI) through the ARDITI-CQM/2018/007-PDG (Fellowship Grant to M.G.), project M1420-01-0145-FEDER-000005-CQM+ (Madeira 14—20).