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dc.creatorTrobos, Margaritaes_ES
dc.creatorFirdaus, Rinintaes_ES
dc.creatorMalchau, Karin Svenssones_ES
dc.creatorTillander, Jonatanes_ES
dc.creatorArnellos, Dimitrioses_ES
dc.creatorRolfson, Olaes_ES
dc.creatorThomsen, Peteres_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.date.accessioned2023-01-11T09:34:06Z
dc.date.available2023-01-11T09:34:06Z
dc.date.issued2022
dc.identifier.citationTrobos, M., Firdaus, R., Svensson Malchau, K., Tillander, J., Arnellos, D., Rolfson, O., Thomsen, P., & Lasa, I. (2022). Genomics of staphylococcus aureus and staphylococcus epidermidis from periprosthetic joint infections and correlation to clinical outcome. Microbiology Spectrum, 10(4), e02181-21. https://doi.org/10.1128/spectrum.02181-21en
dc.identifier.issn2165-0497
dc.identifier.urihttps://hdl.handle.net/2454/44551
dc.description.abstractThe approach of sequencing or genotyping to characterize the pathogenic potential of staphylococci from orthopedic device-related infection (ODRI) has been applied in recent studies. These studies described the genomic carriage of virulence in clinical strains and compared it with those in commensal strains. Only a few studies have directly correlated genomic profiles to patient outcome and phenotypic virulence properties in periprosthetic joint infections (PJIs). We investigated the association between genomic variations and virulence-associated phenotypes (biofilm-forming ability and antimicrobial resistance) in 111 staphylococcal strains isolated from patients with PJI and the infection outcome (resolved/unresolved). The presence of a strong biofilm phenotype in Staphylococcus aureus and an antibiotic-resistant phenotype in Staphylococcus epidermidis were both associated with treatment failure of PJI. In S. epidermidis, multidrug resistance (MDR) and resistance to rifampicin were associated with unresolved infection. Sequence type 45 (ST45) and ST2 were particularly enriched in S. aureus and S. epidermidis, respectively. S. epidermidis ST2 caused the majority of relapses and was associated with MDR and strong biofilm production, whereas ST215 correlated with MDR and non/weak biofilm production. S. aureus agr II correlated with resolved infection, while S. epidermidis agr I was associated with strong biofilm production and agr III with non/weak production. Collectively, our results highlight the importance of careful genomic and phenotypic characterization to anticipate the probability of the strain causing treatment failure in PJI. Due to the high rate of resistant S. epidermidis strains identified, this study provides evidence that the current recommended treatment of rifampicin and a fluoroquinolone should not be administered without knowledge of the resistance pattern.eu
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofMicrobiology Spectrum, 10(4), e02181-21en
dc.rights© 2022 Trobos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAntibiotic resistanceen
dc.subjectBiofilmen
dc.subjectPatient outcomeen
dc.subjectPeriprosthetic joint infectionen
dc.subjectStaphylococcusen
dc.subjectVirulenceen
dc.subjectWhole-genome sequencingen
dc.titleGenomics of Staphylococcus aureus and Staphylococcus epidermidis from periprosthetic joint infections and correlation to clinical outcomeen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.date.updated2023-01-11T09:03:48Z
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1128/spectrum.02181-21
dc.relation.publisherversionhttps://doi.org/10.1128/spectrum.02181-21es_ES
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© 2022 Trobos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Except where otherwise noted, this item's license is described as © 2022 Trobos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

El Repositorio ha recibido la ayuda de la Fundación Española para la Ciencia y la Tecnología para la realización de actividades en el ámbito del fomento de la investigación científica de excelencia, en la Línea 2. Repositorios institucionales (convocatoria 2020-2021).
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