Lossy mode resonance-based optical immunosensor towards detecting gliadin in aqueous solutions
Fecha
2023Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
Impacto
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10.1016/j.foodcont.2023.109624
Resumen
The development of accurate, intuitive, and easy-to-handle devices to detect different types of allergens is on the
rise, as these are useful tools to guarantee consumer safety, which should be a priority for any food industry.
Gliadin, one of the main proteins present in gluten, is the one responsible for triggering the immune system to
produce autoantibodies in celiac disease, the most dange ...
[++]
The development of accurate, intuitive, and easy-to-handle devices to detect different types of allergens is on the
rise, as these are useful tools to guarantee consumer safety, which should be a priority for any food industry.
Gliadin, one of the main proteins present in gluten, is the one responsible for triggering the immune system to
produce autoantibodies in celiac disease, the most dangerous pathology related to gluten. Lossy Mode Resonance
(LMR) based biosensors are lately known as a promising sensing technology and its implementation on planar
waveguides has been shown to result in manageable, sustainable and robust structures. In this work, an LMR
based microfluidic biosensor for gliadin detection is proposed, by coating a coverslip with Titanium Dioxide
(TiO2) by Atomic Layer Deposition (ALD) to generate the resonance phenomena and functionalizing the sensor
surface with anti-gliadin antibody (AGA) through covalent bond. The sensor was exposed to different gliadin
concentrations in ultrapure water, in the range of 0.1–100 ppm with an accuracy of ±0.14 ppm, for a sensitivity
of 1.35 ppm/ml. The calibration curve was obtained from the experimental data corresponding to three repetitions of the assay and a limit of detection (LOD) of 0.05 ppm was achieved. Moreover, the sensor was exposed to
commercial flour samples, some of them labeled as gluten free (GF) and the response agreed with the expected
results according to product label. Biosensor specificity to gliadin was demonstrated by injecting chicken egg
white albumin without obtaining any significant response. [--]
Materias
Gluten,
Gliadin,
Food safety,
Lossy mode resonance,
Biosensor,
Planar waveguide
Editor
Elsevier
Publicado en
Food control 147 (2023) 109624
Departamento
Universidad Pública de Navarra. Departamento de Ingeniería Eléctrica, Electrónica y de Comunicación /
Nafarroako Unibertsitate Publikoa. Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute of Smart Cities - ISC
Versión del editor
Entidades Financiadoras
Authors would like to thank the partial funding by the Spanish Agencia Estatal de Investigacion (AEI) with references PID2019-106070RB-I00 and also by the Public University of Navarra, with the project BIOFOCEL (ref. PJUPNA2033) and a pre-doctoral research grant.