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dc.creatorJiménez Montenegro, Lucíaes_ES
dc.creatorMendizábal Aizpuru, José Antonioes_ES
dc.creatorAlfonso Ruiz, Leopoldoes_ES
dc.creatorAzparren Domínguez, Leirees_ES
dc.creatorUrrutia Vera, Olaiaes_ES
dc.date.accessioned2023-04-20T17:59:59Z
dc.date.available2023-04-20T17:59:59Z
dc.date.issued2022
dc.identifier.citationJiménez-Montenegro, L., Mendizabal, J. A., Alfonso, L., Azparren, L., & Urrutia, O. (2022). Development of a duplex qPCR assay with locked nucleic acid probes for A, B and E kappa-casein variants detection. Scientific Reports, 12(1), 16387. https://doi.org/10.1038/s41598-022-20586-wen
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/2454/45146
dc.description.abstractMilk proteins determine important milk technological characteristics. Among caseins, Ƙ-casein has been correlated with fat and protein content and cheese yield. Fourteen Ƙ-caseins variants have been described but the alleles A, B and E are the most important ones due to their frequency and/or influence on the technological aptitudes of milk. Therefore, in the present study two different duplex qPCR assays with locked nucleic acid probes (for positions 13104 and 13124 of the Ƙ-casein gene) were developed for the detection of A, B and E variants. Firstly, DNA isolation method from milk somatic cells and hair was optimised. The developed 13124-qPCR assay showed an increased sensitivity reaching up to 6.7 copies DNA copies/reaction at a 95% confidence level with A, B and E alleles reference samples. The 13104-qPCR assay reached up to 6.7 DNA copies/reaction for A allele reference sample and 67 DNA copies/reaction for B and E samples. Intra-assay variation results were below 6%. Applicability was determined using DNA samples from animals with known genotype for Ƙ-casein (AA, AB, BB, BE, AE, EE) and both assays were able to discriminate among the six genotypes with 100% accuracy. Thus, this qPCR method represents a sensitive and rapid option for the detection of Ƙ-casein alleles in both hair and milk samples.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherSpringer Natureen
dc.relation.ispartofScientific Reports (2022) 12, 16387en
dc.rights© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDuplex qPCR assayen
dc.subjectLocked nucleic acid probesen
dc.subjectK-caseinen
dc.titleDevelopment of a duplex qPCR assay with locked nucleic acid probes for A, B and E kappa-casein variants detectionen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.date.updated2023-04-20T17:51:33Z
dc.contributor.departmentAgronomía, Biotecnología y Alimentaciónes_ES
dc.contributor.departmentAgronomia, Bioteknologia eta Elikaduraeu
dc.contributor.departmentInstitute on Innovation and Sustainable Development in Food Chain - ISFOODen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1038/s41598-022-20586-w
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-022-20586-w
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License.
Except where otherwise noted, this item's license is described as © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License.

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