Integrated platform for microfluidics based cell culture applications

Abstract

The ongoing developments in the field of microfluidics-based cell culture have led to a significant interest in systems for their visualization and control. The existing cell culture systems are limiting due to cost, complexity, and the need for regular user interventions. This work delves into developing a cost-effective, portable, and automated cell culture and monitoring platform with fluid management and integrated lens free imaging capabilities. The developed system, with a small footprint, weighing approximately 1.7 kg, is highly portable and can be used with existing CO2 incubator systems. With the component cost of the developed system being approximately USD 300, the platform is highly affordable for an integrated cell imaging and automated media replacement system. The system can be used with conventional culture dishes as well as microfluidic systems.