Genome-wide screening of genes whose enhanced expression affects glycogen accumulation in Escherichia coli
Ver/
Fecha
2010Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión aceptada / Onetsi den bertsioa
Impacto
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10.1093/dnares/dsp028
Resumen
Using a systematic and comprehensive gene expression library (the ASKA library), we have carried out a genome-wide screening of the genes whose increased plasmid-directed expression affected glycogen metabolism in Escherichia coli. Of the 4123 clones of the collection, 28 displayed a glycogen-excess phenotype, whereas 58 displayed a glycogen-deficient phenotype. The genes whose enhanced expres ...
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Using a systematic and comprehensive gene expression library (the ASKA library), we have carried out a genome-wide screening of the genes whose increased plasmid-directed expression affected glycogen metabolism in Escherichia coli. Of the 4123 clones of the collection, 28 displayed a glycogen-excess phenotype, whereas 58 displayed a glycogen-deficient phenotype. The genes whose enhanced expression affected glycogen accumulation were classified into various functional categories including carbon sensing, transport and metabolism, general stress and stringent responses, factors determining intercellular communication, aggregative and social behaviour, nitrogen metabolism and energy status. Noteworthy, one-third of them were genes about which little or nothing is known. We propose an integrated metabolic model wherein E. coli glycogen metabolism is highly interconnected with a wide variety of cellular processes and is tightly adjusted to the nutritional and energetic status of the cell. Furthermore, we provide clues about possible biological roles of genes of still unknown functions. [--]
Materias
ASKA library,
Carbohydrate metabolism,
Functional genomics,
ADP glucose pyrophosphorylase,
Adenylate energy charge,
Bacterial,
Glycogen,
Phosphotransferase system,
Tyrosine phosphorylation,
Protein phosphorylation,
Multicellular behavior,
Stringent response,
Carbon metabolism,
Biofilm formation
Editor
Oxford University Press
Publicado en
DNA Research, 2010, 17(2). Págs. 61-71
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This research was partially supported by the grant
BIO2007-63915 from the Comisión Interministerial
de Ciencia y Tecnología and Fondo Europeo de
Desarrollo Regional (Spain) and by Iden
Biotechnology S.L.
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