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dc.creatorRuiz de los Mozos Aliaga, Igores_ES
dc.creatorVergara Irigaray, Martaes_ES
dc.creatorSegura, Víctores_ES
dc.creatorVillanueva San Martín, Maitees_ES
dc.creatorBitarte, Nereaes_ES
dc.creatorSaramago, Margaridaes_ES
dc.creatorDomingues, Susanaes_ES
dc.creatorArraiano, Cecilia M.es_ES
dc.creatorFechter, Pierrees_ES
dc.creatorRomby, Pascalees_ES
dc.creatorValle Turrillas, Jaionees_ES
dc.creatorSolano Goñi, Cristinaes_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.creatorToledo Arana, Alejandroes_ES
dc.date.accessioned2015-12-22T08:50:33Z
dc.date.available2015-12-22T08:50:33Z
dc.date.issued2013
dc.identifier.issn1553-7390 (Print)
dc.identifier.issn1553-7404 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/19768
dc.descriptionUPNa. Instituto de Agrobiotecnología. Laboratorio de Biofilms Microbianos.es_ES
dc.descriptionIncluye 10 ficheros de datoses_ES
dc.description.abstractThe presence of regulatory sequences in the 39 untranslated region (39-UTR) of eukaryotic mRNAs controlling RNA stability and translation efficiency is widely recognized. In contrast, the relevance of 39-UTRs in bacterial mRNA functionality has been disregarded. Here, we report evidences showing that around one-third of the mapped mRNAs of the major human pathogen Staphylococcus aureus carry 39-UTRs longer than 100-nt and thus, potential regulatory functions. We selected the long 39-UTR of icaR, which codes for the repressor of the main exopolysaccharidic compound of the S. aureus biofilm matrix, to evaluate the role that 39-UTRs may play in controlling mRNA expression. We showed that base pairing between the 39- UTR and the Shine-Dalgarno (SD) region of icaR mRNA interferes with the translation initiation complex and generates a double-stranded substrate for RNase III. Deletion or substitution of the motif (UCCCCUG) within icaR 39-UTR was sufficient to abolish this interaction and resulted in the accumulation of IcaR repressor and inhibition of biofilm development. Our findings provide a singular example of a new potential post-transcriptional regulatory mechanism to modulate bacterial gene expression through the interaction of a 39-UTR with the 59-UTR of the same mRNA.en
dc.description.sponsorshipThis work was supported by grants from Spanish Ministry of Economy and Competitiveness (BFU2011-23222, BIO2008-05284-C02-01 and ERA-NET Pathogenomics PIM2010EPA-00606) and from Fundação para a Ciência e Tecnologia - Portugal (ERA-PTG/0002/2010 and PEst-OE/EQB/LA0004/2011). IRdlM and JV were supported by F.P.I. (BES-2009-017410) and Ramón y Cajal (RYC-2009-03948) contracts, respectively, from the Spanish Ministry of Economy and Competitiveness.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPLoS Genetics 9(12): e1004001en
dc.rights© 2013 Ruiz de los Mozos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBase pairing interactionen
dc.subjectStaphylococcus aureusen
dc.subjectmRNA expressionen
dc.subject5-UTRen
dc.subject3-UTRen
dc.subjecticaRen
dc.titleBase pairing interaction between 5′- and 3′-UTRs controls icaR mRNA translation in Staphylococcus aureusen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentIdAB - Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1371/journal.pgen.1004001
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/BFU2011-23222en
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/BIO2008-05284en
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/BIO2008-05284en
dc.relation.publisherversionhttps://dx.doi.org/10.1371/journal.pgen.1004001
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© 2013 Ruiz de los Mozos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as © 2013 Ruiz de los Mozos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.