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dc.creatorMurillo Martínez, Jesúses_ES
dc.creatorKeen, Noel T.es_ES
dc.date.accessioned2017-03-14T12:50:44Z
dc.date.available2017-03-14T12:50:44Z
dc.date.issued1994
dc.identifier.issn0950-382X (Print)
dc.identifier.issn1365-2958 (Electronic)
dc.identifier.urihttp://hdl.handle.net/2454/23889
dc.description.abstractStrain PT23 of Pseudomonas syringae pv, tomato contains four native plasmids, designated A, B, C, and D. By DNA hybridization of genomic and plasmid DNA digests from the wild type and a plasmid-cured strain, we determined that c. 61 kb (c. 74%) of pPT23B is repeated in pPT23A and only c. 17 kb (c. 21%) is in single copy in strain PT23. pPT23B also contains DNA repeated in the chromosome that occurs in three DNA fragments of 0.6, 4.6, and 9.6 kb that might be transposable elements. Additionally, the 9.6 kb fragment also shares sequences with the three other plasmids of strain PT23. By DNA hybridization with the origin of replication from a native plasmid of P. syringae pv. syringae and in vivo replication tests, we identified the origins of replication of plasmids A, B, and D and showed that they cross-hybridize. The putative par region from pPT23 A has also been identified and is not conserved in the other three native plasmids from strain PT23. By using the defined minimal origin of replication from pPT23 A as a probe, we showed that it is highly conserved in 14 strains belonging to nine different pathovars of P. syringae and that as many as five different native plasmids with closely related origins of replication coexist in the same cell. The duplication and reorganization of plasmids might therefore occur at high frequency and could be responsible for the existence of large numbers of native plasmids in P. syringae strains.es_ES
dc.description.sponsorshipJ.M. was supported, in part, by grants 93-258 (DGICYT) and PF90-6984578 from the Ministerio de Educación y Ciencia, Spain. This work was supported by National Science Foundation Grant MCB-9005388-02.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherBlackwell Publishingen
dc.relation.ispartofMolecular Microbiology (1994) 12(6), 941-950en
dc.subjectRepliconen
dc.subjectPFP plasmidsen
dc.subjectrepAen
dc.subjectReplication initiator protein geneen
dc.subjectDuplicated plasmidsen
dc.subjectOrigin of replicationen
dc.subjectPlant pathogenen
dc.titleTwo native plasmids of Pseudomonas syringae pathovar tomato strain PT23 share a large amount of repeated DNA, including replication sequencesen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen


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