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dc.creatorDardouri, Sanaes_ES
dc.creatorChehimi, Soniaes_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.creatorHajlaoui, Mohamed Rabehes_ES
dc.date.accessioned2017-08-11T09:54:26Z
dc.date.available2018-04-01T23:00:12Z
dc.date.issued2017
dc.identifier.issn0035-6441
dc.identifier.urihttps://hdl.handle.net/2454/25081
dc.description.abstractThe present study focused on the molecular characterization of a collection of Erwinia amylovora isolates recovered from different outbreaks in Tunisia between 2012 and 2014. Analysis of 54 isolates, including the reference type strain CFBP 1430, revealed that all Tunisian isolates produced the expected amplicons with diverse primer pairs routinely used for molecular diagnostics of E. amylovora. We also evaluated the genetic variability of these isolates by PCR fingerprinting, using specific primers for clustered regularly interspaced short palindromic repeats (CRISPRs) and for variable number of tandem repeats (VNTR) sequences. For the first method, our results revealed that all the primers used, except those for CRISPR3, which produced an identical amplicon for all isolates, showed some variability among Tunisian isolates. For the second method, forty-nine isolates showed the same fingerprint patterns as the reference type strain CFBP 1430 with all the primers used, whereas four of the isolates showed very divergent patterns. These results suggest that there has been a main introduction of European-type isolates in Tunisia, and possibly a few mutations or other independent introductions of the pathogen. Additionally, these results indicate that PCR fingerprinting using VNTR markers is a most useful tool for discriminating among E. amylovora strains and for their identification in epidemiological studies.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherItalian Phytopathological Societyen
dc.relation.ispartofJournal of Plant Pathology, vol. 99, n. 2 (2017), p. 331-337en
dc.rightsCreative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by-sa/4.0/
dc.subjectFire blighten
dc.subjectErwinia amylovoraen
dc.subjectPCRen
dc.subjectCRIS- PRen
dc.subjectVNTR primersen
dc.subjectFingerprintingen
dc.subjectEpidemiologyen
dc.titleMolecular characterization of Tunisian strains of Erwinia amylovoraen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.4454/jpp.v99i2.3865
dc.relation.publisherversionhttps://dx.doi.org/10.4454/jpp.v99i2.3865
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0)
Except where otherwise noted, this item's license is described as Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0)