Agaricus devoniensis complex comprises a group of heterothallic isolates constituting a basis for breeding
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
A recent phylogenetic reconstruction of Agaricus section Duploannulati revealed
that A. devoniensis and A. subfloccosus are two complexes of species
close to A. bisporus. The A. subfloccosus complex comprises two homothallic
entities, while the A. devoniensis complex was never studied until now. A
sample of 26 isolates, some being unreliably determined, were examined to
(i) confirm their ide ...
[++]
A recent phylogenetic reconstruction of Agaricus section Duploannulati revealed
that A. devoniensis and A. subfloccosus are two complexes of species
close to A. bisporus. The A. subfloccosus complex comprises two homothallic
entities, while the A. devoniensis complex was never studied until now. A
sample of 26 isolates, some being unreliably determined, were examined to
(i) confirm their identity using a PCR-RFLP marker revealing a characteristic
A. devoniensis ITS polymorphism, and (ii) for their ability to fruit in
standard conditions used for A. bisporus cultivation. Twenty one isolates were
confirmed as A. devoniensis, and only two collections from USA were unable
to fruit. The five remaining isolates were excluded from the complex and
were unable to fruit; their ITS1+2 regions were sequenced and alignments
indicated that four of them were similar to A. campestris and that one belonged
to a new entity close to A. bitorquis and A. cappellianus. For the 19
fructifying isolates of the complex, we attempted intrastock and interstock
mating tests with single spore isolates: for three isolates, we did not get spore
germination; and for seven isolates, we observed partial to complete intersterility
between strains. The nine remaining isolates exhibited a unifactorial
system of sexual incompatibility for which eight different mating type alleles
were detected. Within this group, the heterothallic and presumably interfertile
isolates differed in their origin (Greece, France), their habitat (dune,
coniferous trees), and their morphology (mean spore length: 5.6 to 6.6 μm);
they constitute a diversified genetic basis usable to select smooth white and
attractive cultivars for this tasteful edible and cultivable species. [--]
Materias
Agaricus devoniensis
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.