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dc.creatorRodríguez López, Milagroses_ES
dc.creatorBaroja Fernández, Edurnees_ES
dc.creatorZandueta Criado, Aitores_ES
dc.creatorPozueta Romero, Javieres_ES
dc.date.accessioned2018-12-28T09:14:06Z
dc.date.available2018-12-28T09:14:06Z
dc.date.issued2000
dc.identifier.issn0027-8424 (Print)
dc.identifier.issn1091-6490 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31888
dc.description.abstractA distinct phosphodiesterasic activity (EC 3.1.4) was found in both mono- and dicotyledonous plants that catalyzes the hydrolytic breakdown of ADPglucose (ADPG) to produce equimolar amounts of glucose-1-phosphate and AMP. The enzyme responsible for this activity, referred to as ADPG pyrophosphatase (AGPPase), was purified over 1,100-fold from barley leaves and subjected to biochemical characterization. The calculated Keq* (modified equilibrium constant) value for the ADPG hydrolytic reaction at pH 7.0 and 25°C is 110, and its standard-state free-energy change value (DG*) is 22.9 kcalymol (1 kcal 5 4.18 kJ). Kinetic analyses showed that, although AGPPase can hydrolyze several low-molecular weight phosphodiester bond-containing compounds, ADPG proved to be the best substrate (Km 5 0.5 mM). Pi and phosphorylated compounds such as 3-phosphoglycerate, PPi, ATP, ADP, NADP1, and AMP are inhibitors of AGPPase. Subcellular localization studies revealed that AGPPase is localized exclusively in the plastidial compartment of cultured cells of sycamore (Acer pseudoplatanus L.), whereas it occurs both inside and outside the plastid in barley endosperm. In this paper, evidence is presented that shows that AGPPase, whose activity declines concomitantly with the accumulation of starch during development of sink organs, competes with starch synthase (ADPG:1,4-a-D-glucan 4-a- D-glucosyltransferase; EC 2.4.1.21) for ADPG, thus markedly blocking the starch biosynthesis.en
dc.format.extent6 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherNational Academy of Sciencesen
dc.relation.ispartofPNAS July 18, 2000 97 (15) 8705-8710en
dc.rights© The National Academy of Sciencesen
dc.subjectADPG pyrophosphataseen
dc.subjectPhosphodiesteraseen
dc.subjectStarch biosynthesisen
dc.titleAdenosine diphosphate glucose pyrophosphatase: a plastidial phosphodiesterase that prevents starch biosynthesisen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB - Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1073/pnas.120168097
dc.relation.publisherversionhttps://doi.org/10.1073/pnas.120168097
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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