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dc.creatorSerrano García, Amayaes_ES
dc.creatorWilliams, Trevores_ES
dc.creatorSimón de Goñi, Oihanees_ES
dc.creatorLópez Ferber, Migueles_ES
dc.creatorCaballero Murillo, Primitivoes_ES
dc.creatorMuñoz, Deliaes_ES
dc.date.accessioned2019-01-10T08:27:57Z
dc.date.available2019-01-10T08:27:57Z
dc.date.issued2012
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31944
dc.description.abstractA natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack genes that are essential for viral replication or transmission. To determine the likely origins of such genotypically similar population structures, we performed genomic and functional analyses of these genotypes. The homology of nucleotides in the deleted regions was as high as 79%, similar to those of other colinear genomic regions, although some SfMNPV genes were not present in SeMNPV. In addition, no potential consensus sequences were shared between the deletion flanking sequences. These results indicate an evolutionary mechanism that independently generates and sustains deletion mutants within each virus population. Functional analyses using different proportions of complete and deletion genotypes were performed with the two viruses in mixtures of occlusion bodies (OBs) or co-occluded virions. Ratios greater than 3:1 of complete/deletion genotypes resulted in reduced pathogenicity (expressed as median lethal dose), but there were no significant changes in the speed of kill. In contrast, OB yields increased only in the 1:1 mixture. The three phenotypic traits analyzed provide a broader picture of the functional significance of the most extensively deleted SeMNPV genotype and contribute toward the elucidation of the role of such mutants in baculovirus populations.en
dc.description.sponsorshipThis study received financial support from the Spanish Ministry for Science and Technology (AGL2008-05456-C03-01). A.S. received a predoctoral fellowship from the Spanish Ministry of Education and Culture.en
dc.format.extent8 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, February 2013 Volume 79 Number 4, p. 1118–1125en
dc.rights© 2013, American Society for Microbiology. All Rights Reserved.en
dc.subjectSpodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)en
dc.subjectSpodoptera frugiperda MNPV (SfMNPV)en
dc.subjectOcclusion bodies (OBs)en
dc.titleAnalagous population structures for two alphabaculoviruses highlight a functional role for deletion mutantsen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Instituto de Agrobiotecnologíaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutuaeu
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/aem.03021-12
dc.relation.publisherversionhttps://doi.org/10.1128/aem.03021-12
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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