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    Soybean dihydrolipoamide dehydrogenase (ferric leghemoglobin reductase 2) interacts with and reduces ferric rice non-symbiotic hemoglobin 1

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    Date
    2013
    Author
    Gopalasubramaniama, Sabarinathan K. 
    Kondapallib, Kalyan C. 
    Millán-Pacheco, César 
    Pastor, Nina 
    Stemmler, Timothy L. 
    Morán Juez, José Fernando Upna
    Arredondo-Peter, Raúl 
    Version
    Acceso abierto / Sarbide irekia
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    Artículo / Artikulua
    Version
    Versión aceptada / Onetsi den bertsioa
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    Abstract
    Ferrous oxygenated hemoglobins (Hb2+O2) autoxidize to ferric Hb3+, but Hb3+ is reduced to Hb2+ by enzymatic and non-enzymatic mechanisms. We characterized the interaction between the soybean ferric leghemoglobin reductase 2 (FLbR2) and ferric rice non-symbiotic Hb1 (Hb13+). Spectroscopic analysis showed that FLbR2 reduces Hb13+. Analysis by tryptophan fluorescence quenching showed that FLbR2 i ... [++]
    Ferrous oxygenated hemoglobins (Hb2+O2) autoxidize to ferric Hb3+, but Hb3+ is reduced to Hb2+ by enzymatic and non-enzymatic mechanisms. We characterized the interaction between the soybean ferric leghemoglobin reductase 2 (FLbR2) and ferric rice non-symbiotic Hb1 (Hb13+). Spectroscopic analysis showed that FLbR2 reduces Hb13+. Analysis by tryptophan fluorescence quenching showed that FLbR2 interacts with Hb13+, however the use of ITC and IEF techniques revealed that this interaction is weak. In silico modeling showed that predicted FLbR2 and native Hb13+ interact at the FAD-binding domain of FLbR2 and the CD-loop and helix F of Hb13+. [--]
    Subject
    Fluorescence quenching, Hemoglobin, Isothermal calorimetry, Non-symbiotic, Oryza sativa, Protein-protein interaction
     
    Publisher
    Simplex Academic Publishers
    Published in
    ScienceJet 2013, 2: 33
    Departament
    Universidad Pública de Navarra. Departamento de Ciencias del Medio Natural / Nafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Saila
     
    URI
    https://hdl.handle.net/2454/33266
    Sponsorship
    This work was funded by CoNaCyT (project no. 42873Q), México (to R. A.-P.), Dept. of Education, Government of Navarre (no. 999–2006), Spain (to J. F. M.) and NIDDKD (DK068139), USA (to T. L. S.).
    Appears in Collections
    • Artículos de revista - Aldizkari artikuluak [2924]
    • Artículos de revista DCMN - NIZS Aldizkari artikuluak [85]
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