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dc.creatorVarela Martínez, Endikaes_ES
dc.creatorAbendaño, Naiaraes_ES
dc.creatorAsín, Javieres_ES
dc.creatorSistiaga Poveda, Maialenes_ES
dc.creatorPérez, Marta Maríaes_ES
dc.creatorReina Arias, Ramséses_ES
dc.creatorAndrés Cara, Damián dees_ES
dc.creatorLuján, Lluíses_ES
dc.creatorJugo, Begoña M.es_ES
dc.date.accessioned2019-07-17T12:13:13Z
dc.date.available2019-07-17T12:13:13Z
dc.date.issued2018
dc.identifier.issn1664-3224
dc.identifier.urihttps://hdl.handle.net/2454/33615
dc.description.abstractThere have been few in vivo studies on the effect of aluminum hydroxide adjuvant and its influence on the immune response to vaccination. In this study, lambs received a parallel subcutaneous treatment with either commercial vaccines containing aluminum hydroxide or an equivalent dose of this compound only with the aim of identifying the activated molecular signature. Blood samples were taken from each animal at the beginning and at the end of the experiment and PBMCs isolated. Total RNA and miRNA libraries were prepared and sequenced. After alignment to the Oar3.1 reference genome and differential expression with 3 programs, gene enrichment modeling was performed. For miRNAs, miRBase and RNAcentral databases were used for detection and characterization. Three expression comparisons were made: vaccinated animals at the beginning and at the end of the treatment, adjuvanted animals at the same times, and animals of both treatments at the end of the experiment. After exposure to both treatments, a total of 2,473; 2,980 and 429 differentially expressed genes were identified in vaccinated animals, adjuvanted animals and animals at the end of both treatments, respectively. In both adjuvant and vaccine treated animals the NF-κB signaling pathway was enriched. On the other hand, it can be observed a downregulation of cytokines and cytokine receptors in the adjuvanted group compared to the vaccinated group at the final time, suggesting a milder induction of the immune response when the adjuvant is alone. As for the miRNA analysis, 95 miRNAs were detected: 64 previously annotated in Ovis aries, 11 annotated in Bos taurus and 20 newly described. Interestingly, 6 miRNAs were differentially expressed in adjuvant treated animals, and 3 and 1 in the other two comparisons. Lastly, an integrated miRNA-mRNA expression profile was developed, in which a miRNA-mediated regulation of genes related to DNA damage stimulus was observed. In brief, it seems that aluminum-containing adjuvants are not simple delivery vehicles for antigens, but also induce endogenous danger signals that can stimulate the immune system. Whether this contributes to long-lasting immune activation or to the overstimulation of the immune system remains to be elucidated.en
dc.description.sponsorshipThis work was supported by a MINECO project grant (AGL2013-49137-C3-3-R to BJ and AGL2013-49137-C3-2-R to LL and AGL2013-49137-C3-1 to DdA), a predoctoral fellowship from the UPV/EHU to EV-M (PIF15/361) and a postdoctoral fellowship from the UPV/EHU to NA (ESPDOC16/43).en
dc.format.extent16 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Immunology, 9:2406en
dc.rights© 2018 Varela-Martínez, Abendaño, Asín, Sistiaga-Poveda, Pérez, Reina, de Andrés, Luján and Jugo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAdjuvanten
dc.subjectAluminum hydroxideen
dc.subjectMiRNAen
dc.subjectPBMCsen
dc.subjectRNA-seqen
dc.subjectSheepen
dc.subjectTranscriptomeen
dc.subjectVaccinationen
dc.titleMolecular signature of aluminum hydroxide adjuvant in ovine PBMCs by integrated mRNA and microRNA transcriptome sequencingen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.3389/fimmu.2018.02406
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/AGL2013-49137en
dc.relation.publisherversionhttps://doi.org/10.3389/fimmu.2018.02406
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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© 2018 Varela-Martínez, Abendaño, Asín, Sistiaga-Poveda, Pérez, Reina,
de Andrés, Luján and Jugo. This is an open-access article distributed under the
terms of the Creative Commons Attribution License (CC BY). The use, distribution
or reproduction in other forums is permitted, provided the original author(s) and
the copyright owner(s) are credited and that the original publication in this journal
is cited, in accordance with accepted academic practice. No use, distribution or
reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as © 2018 Varela-Martínez, Abendaño, Asín, Sistiaga-Poveda, Pérez, Reina, de Andrés, Luján and Jugo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.