Expression and purification of recombinant phytoglobins and their role in the oxidative pathways for the production of reactive N species in plants

Abstract

Reactive N species (RNS) are reactive molecules that plays a very wide role in many biological organisms. Plants are one of them where its action have been reported in numerous processes such as breaking dormancy and seed germination, control of stomatal a perture, plant defense against fungal infections or interaction with antioxidants and prooxidants to form other reactive compounds. Reactive N species are considered signaling molecules due to the capacity of binding to certain species of proteins to triggering physiological responses. The use of proteomics has been successfully used to determine proteins that may react directly with signaling compounds, including RNS. This research is focused on the production and purification of recombinant proteins, and their characterization as catalytic mediators in the oxidative pathway for the production of reactive N species in plants.