Quantification of dose-mortality responses in adult Diptera: validation using Ceratitis capitata and Drosophila suzukii responses to spinosad
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Artículo / Artikulua
Versión publicada / Argitaratu den bertsioa
Quantitative laboratory bioassay methods are required to evaluate the toxicity of novel insecticidal compounds for pest control and to determine the presence of resistance traits. We used a radioactive tracer based on P-32-ATP to estimate the volume of a droplet ingested by two dipteran pests: Ceratitis capitata (Tephritidae) and Drosophila suzukii (Drosophilidae). Using blue food dye it was poss ... [++]
Quantitative laboratory bioassay methods are required to evaluate the toxicity of novel insecticidal compounds for pest control and to determine the presence of resistance traits. We used a radioactive tracer based on P-32-ATP to estimate the volume of a droplet ingested by two dipteran pests: Ceratitis capitata (Tephritidae) and Drosophila suzukii (Drosophilidae). Using blue food dye it was possible to distinguish between individuals that ingested the solution from those that did not. The average volume ingested by C. capitata adults was 1.968 mu l. Females ingested a similar to 20% greater volume of solution than males. Adults of D. suzukii ingested an average of 0.879 mu l and females ingested similar to 30% greater volume than males. The droplet feeding method was validated using the naturally-derived insecticide spinosad as the active ingredient (a.i.). For C. capitata, the concentration-mortality response did not differ between the sexes or among three different batches of insects. Lethal dose values were calculated based on mean ingested volumes. For C. capitata LD50 values were 1.462 and 1.502 ng a.i./insect for males and females, respectively, equivalent to 0.274 and 0.271 ng a.i./mg for males and females, respectively, when sex-specific variation in body weight was considered. Using the same process for D. suzukii, the LD50 value was estimated at 2.927 ng a.i./insect, or 1.994 ng a.i./mg based on a mean body weight of 1.67 mg for both sexes together. We conclude that this technique could be readily employed for determination of the resistance status and dose-mortality responses of insecticidal compounds in many species of pestiferous Diptera. [--]
Public Library of Science
Plos One, 14(2): e0210545
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa. IMAB - Institute for Multidisciplinary Research in Applied Biology / Universidad Pública de Navarra. Departamento de Agronomía, Biotecnología y Alimentación / Nafarroako Unibertsitate Publikoa. Agronomia, Bioteknologia eta Elikadura Saila
The study was funded by the Ministerio de Economía y Competitividad (MEC), Spain, projects AGL2014-57752-C2-1-R and AGL2017-83498-C2-1-R awarded to PC and TW. DDdL received a student scholarship from MEC project AGL2014-57752-C2-1-R. MV and IMM were funded by Gobierno de Navarra, Spain, project no. BTMOL-PI028. JC received a predoctoral scholarship from the Universidad Publica de Navarra, Spain.
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