The CCAAT box-binding transcription factor NF-YA1 controls rhizobial infection
Acceso abierto / Sarbide irekia
Artículo / Artikulua
Versión publicada / Argitaratu den bertsioa
Symbiosis between legume plants and soil rhizobia culminates in the formation of a novel root organ, the 'nodule', containing bacteria differentiated as facultative nitrogen-fixing organelles. MtNF-YA1 is a Medicago truncatula CCAAT box-binding transcription factor (TF), formerly called HAP2-1, highly expressed in mature nodules and required for nodule meristem function and persistence. Here a ro ... [++]
Symbiosis between legume plants and soil rhizobia culminates in the formation of a novel root organ, the 'nodule', containing bacteria differentiated as facultative nitrogen-fixing organelles. MtNF-YA1 is a Medicago truncatula CCAAT box-binding transcription factor (TF), formerly called HAP2-1, highly expressed in mature nodules and required for nodule meristem function and persistence. Here a role for MtNF-YA1 during early nodule development is demonstrated. Detailed expression analysis based on RNA sequencing, quantitiative real-time PCR (qRT-PCR), as well as promoter–β-glucuronidase (GUS) fusions reveal that MtNF-YA1 is first induced at the onset of symbiotic development during preparation for, and initiation and progression of, symbiotic infection. Moreover, using a new knock-out mutant, Mtnf-ya1-1, it is shown that MtNF-YA1 controls infection thread (IT) progression from initial root infection through colonization of nodule tissues. Extensive confocal and electronic microscopic observations suggest that the bulbous and erratic IT growth phenotypes observed in Mtnf-ya1-1 could be a consequence of the fact that walls of ITs in this mutant are thinner and less coherent than in the wild type. It is proposed that MtNF-YA1 controls rhizobial infection progression by regulating the formation and the wall of ITs. [--]
Oxford University Press
Journal of Experimental Botany, 2014, 65(2), 481-494
Universidad Pública de Navarra. Departamento de Ciencias del Medio Natural / Nafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Saila
This work was funded by the ANR-09-BLAN-0033-01 HAPIHUB project, including a post-doctoral grant to PL. This work is part of the 'Laboratoire d'Excellence' (LABEX) entitled TULIP (ANR-10-LABX-41). The Mtnf-ya1-1 mutant was identified by a TILLING approach within a A17 Jemalong EMS-mutagenized population, in the frame of the FP6-GLIP (Grain Legume Integrated Project) project. EL is a recipient of the Marie Curie International Outgoing Fellowships for Career Development within the 7th European Union Framework Program (FP7-PEOPLE-2009). This work was carried out with the support of the 'Cooperative Research Program for Agricultural Science & Technology Development (Project no. PJ906910)', Rural Development Administration, Republic of Korea, to JHM and DRC.
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