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dc.creatorMoleres Apilluelo, Javieres_ES
dc.creatorSantos López, Alfonsoes_ES
dc.creatorLázaro, Isidroes_ES
dc.creatorLabairu, Javieres_ES
dc.creatorPrat, Cristinaes_ES
dc.creatorArdanuy, Carmenes_ES
dc.creatorGonzález Zorn, Brunoes_ES
dc.creatorAragon, Virginiaes_ES
dc.creatorGarmendia García, Juncales_ES
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.description.abstractHaemophilus parasuis, the causative agent of Glässer’s disease, is one of the early colonizers of the nasal mucosa of piglets. It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H. parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the blaROB-1 β-lactamase was detected in eight colonizing strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable, and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glässer’s disease or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir of β-lactam resistance genes which can be transferred to pathogens or other bacteria.en
dc.description.sponsorshipJ.M. is funded by Ph.D. studentship BES-2013-062644 from the Ministerio de Economía y Competitividad-MINECO, Spain. This work has been funded by grants from MINECO (SAF2012-31166) and the Departamento Industria Gobierno Navarra (IlQ14064.R12) to J.G., MINECO (AGL2013-45662) to V.A., and EU projects EvoTAR 282004-FP7 and EFFORT 613754-FP7 to B.G.-Z.en
dc.format.extent13 p.
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, May 2015 Volume 81 Number 9en
dc.rights© 2015, American Society for Microbiology. All Rights Reserved.en
dc.subjectHaemophilus parasuisen
dc.titleNovel blaROB-1-bearing plasmid conferring resistance to β-lactams in Haemophilus parasuis isolates from healthy weaning pigsen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/FP7/282004en
dc.relation.projectIDinfo:eu-repo/grantAgreement/European Commission/FP7/613754en
dc.type.versionVersión publicada / Argitaratu den bertsioaes

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