AtCube: performing pathogen-root infection tests on Arabidopsis thaliana in a completely controlled way
Fecha
2022Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1016/j.pmpp.2021.101780
Resumen
Arabidopsis thaliana represents the main model plant for the experimental analysis of plant genetics and developmental biology of photoautotrophic organisms, due to characteristics such as size, life cycle, fecundity, genetics and its easy experimental manipulation. In turn, A. thaliana is used as a model plant in plant-microorganism interaction studies, being of great importance in the knowledge ...
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Arabidopsis thaliana represents the main model plant for the experimental analysis of plant genetics and developmental biology of photoautotrophic organisms, due to characteristics such as size, life cycle, fecundity, genetics and its easy experimental manipulation. In turn, A. thaliana is used as a model plant in plant-microorganism interaction studies, being of great importance in the knowledge of the form of infection of numerous plant pathogens. The present work is based on the development of an A. thaliana plant growth system in Phytatray II boxes and culture substrate, trying to establish a system that is free of contamination, completely controlled, self-sufficient, reproducible and standardized, called AtCube. Through the use of different necrotrophic and biotrophic/hemibiotrophic root-pathogens, bacteria, fungi and oomycetes, and the comparison with a conventional system of growth in plots, various results are analyzed on the effects of pathogens on plants and the advantages and differences of the AtCube system. This work makes it possible to highlight that the AtCube system represents a methodology that allows obtaining results similar to other systems but with important advantages regarding its standardization, rigor and reproducibility with respect to studies with A. thaliana and root pathogens. Furthermore, it could represent an equally efficient system in studies with other plant species and the application of foliar pathogens and/or beneficial microorganisms. [--]
Materias
Arabidopsis thaliana,
Defense-related genes,
Growth chamber,
Reactive oxygen species,
Root-pathogen
Editor
Elsevier
Publicado en
Physiological and Molecular Plant Pathology, 117 (2022) 101780
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Multidisciplinary Research in Applied Biology - IMAB
Versión del editor
Entidades Financiadoras
This work was supported by the Instituto de Estudios Zamoranos 'Florián de Ocampo' (IEZFO), in the XXVII and XXVIII Call for Research Grants, corresponding to the years 2019 and 2020.