Multiple relaxases contribute to the horizontal transfer of the virulence plasmids from the tumorigenic bacterium Pseudomonas syringae pv. savastanoi NCPPB 3335
Fecha
2022Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
Impacto
|
10.3389/fmicb.2022.1076710
Resumen
Pseudomonas syringae pv. savastanoi NCPPB 3335 is the causal agent of
olive knot disease and contains three virulence plasmids: pPsv48A (pA), 80 kb;
pPsv48B (pB), 45 kb, and pPsv48C (pC), 42 kb. Here we show that pB contains a
complete MPFT (previously type IVA secretion system) and a functional origin
of conjugational transfer adjacent to a relaxase of the MOBP family; pC also
contains a fu ...
[++]
Pseudomonas syringae pv. savastanoi NCPPB 3335 is the causal agent of
olive knot disease and contains three virulence plasmids: pPsv48A (pA), 80 kb;
pPsv48B (pB), 45 kb, and pPsv48C (pC), 42 kb. Here we show that pB contains a
complete MPFT (previously type IVA secretion system) and a functional origin
of conjugational transfer adjacent to a relaxase of the MOBP family; pC also
contains a functional oriT-MOBP array, whereas pA contains an incomplete
MPFI (previously type IVB secretion system), but not a recognizable oriT.
Plasmid transfer occurred on solid and in liquid media, and on leaf surfaces
of a non-host plant (Phaseolus vulgaris) with high (pB) or moderate frequency
(pC); pA was transferred only occasionally after cointegration with pB. We
found three plasmid-borne and three chromosomal relaxase genes, although
the chromosomal relaxases did not contribute to plasmid dissemination.
The MOBP relaxase genes of pB and pC were functionally interchangeable,
although with di ering eciencies. We also identified a functional MOBQ
mobilization region in pC, which could only mobilize this plasmid. Plasmid
pB could be eciently transferred to strains of six phylogroups of P. syringae
sensu lato, whereas pC could only be mobilized to two strains of phylogroup 3
(genomospecies 2). In two of the recipient strains, pB was stably maintained
after 21 subcultures in liquid medium. The carriage of several relaxases
by the native plasmids of P. syringae impacts their transfer frequency and,
by providing functional diversity and redundancy, adds robustness to the
conjugation system. [--]
Materias
Cytokinin biosynthesis,
Type III effectors,
Olive knot disease,
Type IV secretion system,
Mating pair formation,
Pseudomonas amygdali,
Pseudomonas savastanoi,
Conjugation (mating)
Editor
Frontiers Media
Publicado en
Frontiers in Microbiology 13:1076710
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Multidisciplinary Research in Applied Biology - IMAB
Versión del editor
Entidades Financiadoras
This work was funded by the Spanish Plan Nacional
I+D+i grants PID2020-115177RB-C21 and PID2020-
115177RB-C22 financed by the Ministerio de Ciencia
e Innovación and the Agencia Estatal de Investigación,
MCIN/AEI/10.13039/501100011033/, Spain.
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