Bacillus thuringiensis Cyt proteins as enablers of activity of Cry and Tpp toxins against Aedes albopictus
Fecha
2023Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
Impacto
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10.3390/toxins15030211
Resumen
Aedes albopictus is a species of mosquito, originally from Southeast Asia, that belongs to the Culicidae family and the Dipteran insect order. The distribution of this vector has rapidly changed over the past decade, making most of the temperate territories in the world vulnerable to important human vector-borne diseases such as dengue, yellow fever, zika or chikungunya. Bacillus thuringiensis va ...
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Aedes albopictus is a species of mosquito, originally from Southeast Asia, that belongs to the Culicidae family and the Dipteran insect order. The distribution of this vector has rapidly changed over the past decade, making most of the temperate territories in the world vulnerable to important human vector-borne diseases such as dengue, yellow fever, zika or chikungunya. Bacillus thuringiensis var. israeliensis (Bti)-based insecticides represent a realistic alternative to the most common synthetic insecticides for the control of mosquito larvae. However, several studies have revealed emerging resistances to the major Bti Crystal proteins such as Cry4Aa, Cry4Ba and Cry11Aa, making the finding of new toxins necessary to diminish the exposure to the same toxicity factors overtime. Here, we characterized the individual activity of Cyt1Aa, Cry4Aa, Cry4Ba and Cry11Aa against A. albopictus and found a new protein, Cyt1A-like, that increases the activity of Cry11Aa more than 20-fold. Additionally, we demonstrated that Cyt1A-like facilitates the activity three new Bti toxins: Cry53-like, Cry56A-like and Tpp36-like. All in all, these results provide alternatives to the currently available Bti products for the control of mosquito populations and position Cyt proteins as enablers of activity for otherwise non-active crystal proteins. [--]
Materias
Aedes albopictus,
Bacillus thuringiensis,
Cry toxins,
Cyt toxins,
Mosquitocidal,
Synergy,
Tpp toxins
Publicado en
Toxins 2023, 15(3), 211
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Multidisciplinary Research in Applied Biology - IMAB
Versión del editor
Entidades Financiadoras
This research was funded by Gobierno de Navarra with grant numbers 0011-1365-2020-000297 to C.J.C. and 0011-1365-2020-000033 to A.T.-A. L.L. received a doctoral grant from the European Union’s H2020 research and innovation programme under Marie Sklodowska-Curiegrant agreement Nº801586. A.M.-G. was supported by a FPU PhD grant from the Spanish Ministry of Science and Innovation (FPU20/05496). A.B. Fernández’s contract was cofounded by the Program Torres Quevedo (Ministerio de Ciencia, Innovación y Universidades, Gobierno de España) (PTQ-2018-010091) and Bioinsectis SL.