Femtomolar detection by nanocoated fiber label-free biosensors
Fecha
2018Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión aceptada / Onetsi den bertsioa
Identificador del proyecto
AEI//TEC2016-78047-R AEI//TEC2016-79367-C2-2-R Gobierno de Navarra//72%2F2015
Impacto
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10.1021/acssensors.7b00918
Resumen
The advent of optical fibre-based biosensors combined with that of nanotechnologies has provided an oppor-tunity for developing in situ, portable, lightweight, versatile and high-performance optical sensing platforms. We report on the generation of lossy mode resonances by the deposition of nm-thick metal oxide films on optical fibres, which makes it possible to measure precisely and accurately t ...
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The advent of optical fibre-based biosensors combined with that of nanotechnologies has provided an oppor-tunity for developing in situ, portable, lightweight, versatile and high-performance optical sensing platforms. We report on the generation of lossy mode resonances by the deposition of nm-thick metal oxide films on optical fibres, which makes it possible to measure precisely and accurately the changes in optical properties of the fibre-surrounding medium with very high sensitivity compared to other technology platforms, such as long period gratings or surface plasmon resonances, the gold standard in label-free and real-time biomolecular interaction analysis. This property, combined with the application of specialty structures such as D-shaped fibres, permits enhancing the light-matter interaction. SEM and TEM imaging together with X-EDS tool have been utilised to characterise the two films used, i.e. indium tin oxide and tin dioxide. More-over, the experimental transmission spectra obtained after the deposition of the nanocoatings have been numerically cor-roborated by means of wave propagation methods. With the use of a conventional wavelength interrogation system and ad-hoc developed microfluidics, the shift of the lossy mode resonance can be reliably recorded in response to very low analyte concentrations. Repeated experiments confirm a big leap in performance thanks to the capability to detect femtomolar concentrations in human serum, improving the detection limit by three orders of magnitude when compared with other fibre-based configurations. The biosensor has been regenerated several times by injecting sodium dodecyl sul-phate, which proves the capability of sensor to be reused. [--]
Materias
Optical biosensor,
Lossy mode resonance,
Optical fiber sensor,
Nanometric metal oxide film,
Label-free biomolecular interaction,
Femtomolar detection limit,
Regeneration
Editor
ACS
Publicado en
ACS Sensors, 2018, 3, 936-943
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute of Smart Cities - ISC /
Universidad Pública de Navarra. Departamento de Ingeniería Eléctrica y Electrónica /
Nafarroako Unibertsitate Publikoa. Ingeniaritza Elektrikoa eta Elektronikoa Saila
Versión del editor
Entidades Financiadoras
This work was supported by the European Community for theproject Hemospec (FP7-611682), by the National ResearchCouncil of Italy (CNR) for the Short Term Mobility program2017, by the Government of Navarra (project no. 72/2015)and by the Spanish Agencia Estatal de Investigación (AEI) andEuropean Regional Development Fund (FEDER) (TEC2016-78047-R, TEC2016-79367-C2-2-R).